New findings in the vaginal microbiome

New findings in the vaginal microbiome
Schematic representation of the sampling scheme and taxonomic profiles. Over 50 women were recruited using one of three contraceptive regimens: NHC (non-hormonal contraceptives), COC (combined oral contraceptives) and LNG-IUS (levonorgestrel intrauterine system). Included women were sampled at the hospital during the menstrual phase (cycle days 1–3), follicular phase (cycle days 8–12) and luteal phase (cycle days 18–22). Blood samples, saliva and rectal swabs were collected at the hospital; vaginal swabs and faeces were collected by the women at home. Women on LNG-IUS with oligo-/amenorrhoea started their sampling at a random day. The average taxonomic profile observed at each body site is depicted as circle charts. Only the vaginal swabs are divided by contraceptive and sampling point in this figure, since they are the only ones with clear visible differences. Credit: Human Reproduction (2022). DOI: 10.1093/humrep/deac094

A new study published in Human Reproduction shows that the menstrual phase has effects on the microbiome. "This will be very important for planning future studies, both in basic science and for interventions aiming to improve the vaginal microbiome," corresponding author Ina Schuppe Koistinen at the Department of Microbiology, Tumor and Cell biology at Karolinska Institutet, says.

The use of oral contraceptives or intrauterine system has not been associated with the or . In this new study however, an increased diversity with more , is observed during menses. During the follicular and luteal phases, the study shows that there is an expansion of the proportion of Lactobacillus, which are considered the most healthy species in the vaginal microbiome. It also correlates with measured serum oestradiol levels. During menses, 58% of the women have a suboptimal vaginal microbiome, but it declines to 32% in the follicular phase, down to 29% in the luteal phase.

The bacterial diversity in saliva is lowest during menses, and no differences were found for the fecal microbiome. Only the vaginal microbiome presents a clear cluster structure with dominance of one out of four bacterial taxa: Lactobacillus crispatus, Lactobacillus iners, Gardnerella vaginalis or Prevotella spp. The other body sites showed all possible mixtures of bacteria. Comparing samples from the same individual with samples from other individuals, the microbiome is more similar within the same person, despite the effects of menstrual cycle or hormonal treatment.

"The human microbiome has been implicated in many different areas of medicine, from cancer to . Knowing that very commonly used contraceptives do not affect the microbiome is a positive finding," Ina Schuppe Koistinen says. "The vaginal microbiome in particular has also been connected to many gynecological outcomes, such as STI acquisition, the development of cervical cancer and likelihood of pregnancy following IVF. For this field, knowing that the menstrual phase has a major effect on the microbiome will be very important for planning future studies, both in basic science and for interventions aiming to improve the ."

More information: Maria Christine Krog et al, The healthy female microbiome across body sites: effect of hormonal contraceptives and the menstrual cycle, Human Reproduction (2022). DOI: 10.1093/humrep/deac094

Journal information: Human Reproduction

Citation: New findings in the vaginal microbiome (2022, May 13) retrieved 28 September 2023 from
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.

Explore further

Just don't douche: What your vaginal biome can tell you about your health and pregnancy


Feedback to editors