Structure of antibodies could be key to more effective cancer treatments

Scientists analyze structure of antibodies that could be key to more effective cancer treatments
The flexible hinge allows the two antibody arms to move freely, leading to weak receptor activity. Credit: University of Southampton

Researchers at the University of Southampton have gained unprecedented new insight into the key properties of an antibody needed to fight off cancer.

The , published in Science Immunology, revealed how changing the flexibility of the antibody could stimulate a stronger immune response.

The findings have enabled the Southampton team to design antibodies to activate important receptors on to "fire them up" and deliver more powerful anti-cancer effects.

The scientists believe their findings could pave the way to improve antibody drugs that target cancer as well as other autoimmune diseases.

In the study, the team investigated antibody drugs targeting the receptor CD40 for cancer treatment. Clinical development has been hampered by a lack of understanding of how to stimulate the receptors to the right level. The problem being that if antibodies are too active they can become toxic.

Previous Southampton research has shown that a specific type of antibody called IgG2 is uniquely suited as a template for pharmaceutical intervention, since it is more active than other antibody types. However, the reason why it is more active had not been determined.

What was known, however, is that the structure between the antibody arms, the so called hinges, changes over time.

Scientists analyze structure of antibodies that could be key to more effective cancer treatments
The rigid hinge, with its disulfide links (yellow), keeps the two antibody arms constrained, leading to stronger receptor activity. Credit: University of Southampton

This latest research harnesses this property of the hinge and explains how it works: the researchers call this process "disulfide-switching."

In their study, the Southampton team analyzed the effect of modifying the hinge and used a combination of biological activity assays, , and to study how disulfide switching alters antibody structure and activity.

Dr. Ivo Tews, Associate Professor in Structural Biology at the University of Southampton, said, "Our approach was to analyze the structure of the antibody in atomic detail, using the method of X-ray crystallography. While the resulting picture is very accurate, the information on how they move their 'arms' is missing, and we needed an image of the antibody in solution, for which we used an X-ray scattering approach called SAXS. We then used mathematical models and a chemical-computing approach to analyze the data, using the Southampton High Performance Computing cluster IRIDIS."

Through this detailed study of the hinge the team revealed that more compact, rigid antibodies are more active than their flexible counterparts.

Professor Mark Cragg, of the Center for Cancer Immunology at the University of Southampton, said, "This study has given us new information about how to engineer antibodies to deliver a better immune response. We propose that more rigid antibodies enable the receptors to be bound closer together on the , promoting receptor clustering and stronger signaling for activity. This means by modifying the hinge we can now generate more or less active antibodies in a more predictable way.

"Excitingly, our findings could have wider implications as it may provide a highly controlled and tractable means of developing antibodies for in future immunostimulatory antibody drugs."


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More information: Christian M. Orr et al, Hinge disulfides in human IgG2 CD40 antibodies modulate receptor signaling by regulation of conformation and flexibility, Science Immunology (2022). DOI: 10.1126/sciimmunol.abm3723. www.science.org/doi/10.1126/sciimmunol.abm3723
Journal information: Science Immunology

Citation: Structure of antibodies could be key to more effective cancer treatments (2022, July 8) retrieved 6 October 2022 from https://medicalxpress.com/news/2022-07-antibodies-key-effective-cancer-treatments.html
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