Validation of continuous theta-burst stimulation as a way to reduce neuronal excitability

Validation of continuous theta-burst stimulation as a way to reduce neuronal excitability
Brain targeting and stimulation protocol. (A) Left: Anatomical magnetic resonance image performed with a dummy coil, reproducing the position and angle of the Magstim coil during recordings (45°ee angle with respect to the vertical). Right: Coronal view of the monkey brain indicating the location of the TMS coil with respect to parietal area PFG. The red, dashed line indicates the trajectory of the electrode during recordings. For every experiment, a D25 mm figure-of-eight TMS coil (in black) was rigidly anchored to the monkey’s implant. (B) Three-dimensional models of the monkey’s skull and implant (left: top view, right: lateral view). Prior to the experiment, two guiding rods were attached to the monkey’s head implant based on MRI estimations of the cortical target coordinates. This allowed a precise and reproducible coil positioning across experimental sessions. (C) Example of the raw signal recorded during a typical stimulation session. The high voltage, saturated peaks indicate the stimulation time stamps. TMS was administered in three different epochs corresponding, in this order, to: single-pulse TMS (sTMS) applied at light onset (20 min), cTBS (20 s), and again, sTMS (60–120 min). cTBS, continuous theta-burst stimulation; MRI, magnetic resonance imaging; TMS, transcranial magnetic stimulation. Credit: eLife (2022). DOI: 10.7554/eLife.65536

Transcranial magnetic stimulation, a form of non-invasive brain stimulation, has been used for the past 35 years to investigate brain function in health and disease.

Applied to quantify causal changes in during movement performance and cognitive behaviors, the technique has led to the definition of a detailed causal brain map underpinning each particular aspect of human behavior. Increased knowledge of the technique's effectiveness will allow for its wider use in fundamental research and clinical applications, with the potential to improve our understanding of the relationship between and behavior.

Continuous theta-burst stimulation (cTBS), a variant of , has subsequently become a standard non-invasive technique to induce offline changes in cortical excitability in human volunteers. Yet cTBS suffers from a high variability across volunteers and its exact effects on neurons in awake behaving animals has never been described.

A team of researchers at King's College London and KU Leuven, led by Dr. Marco Davare in the School of Life Course & Population Sciences, recorded for the first time individual neurons in macaque before and after 20 seconds of cTBS. Their findings, published today by eLife, confirm that cTBS is an accurate way to measure the impact of neuron activity on behavior.

The researchers found that, overall, cTBS induced a marked reduction in neuronal excitability, which grew over time consistent with previous studies in humans. They observed similar effects on the baseline activity, on task-related activity and on behavior. Interestingly, a subset of the neurons also showed temporary hyperexcitability during the recovery phase.

CTBS works by non-invasively and very transiently inducing a "virtual lesion" in a small brain region, allowing researchers to measure the behavioral deficits in healthy volunteers or patients in a given cognitive (for example: decision-making) or motor (for example: grasping) task. By studying the behavioral deficits induced, one can then deduce the causal role of the brain region targeted by TBS.

This study is part of a series of investigations aimed at validating a host of neuromodulation techniques used in healthy human volunteers and patients in the animal model closest to the human. By providing the first experimental evidence of the effects of cTBS on single neurons in awake behaving monkeys, the researchers shed new light on the reasons underlying cTBS variability—findings that prove seminal for the interpretation of all TMS studies on the relationship between brain function and behavior.

More information: Maria C Romero et al, Neural effects of continuous theta-burst stimulation in macaque parietal neurons, eLife (2022). DOI: 10.7554/eLife.65536
Journal information: eLife

Citation: Validation of continuous theta-burst stimulation as a way to reduce neuronal excitability (2022, September 13) retrieved 8 December 2022 from
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