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Scientists publish recipe for making blood vessel cells from patient stem cells

Scientists publish recipe for making blood vessel cells from patient stem cells
Graphical abstract. Credit: STAR Protocols (2023). DOI: 10.1016/j.xpro.2023.102292

Researchers at NIH's National Eye Institute have published a detailed protocol for making three cell types that are key components to form blood vessels and capillaries. The technique enables researchers to make tissues for study and potentially for future treatments.

"This protocol generates , pericytes, and fibroblasts from human induced pluripotent stem cells called iPSCs," said Tea Soon Park, Ph.D., NEI Ocular and Stem Cell Translational Research Section, and lead author of the protocol.

IPSCs are made in a lab, starting with skin or . They can then be modified to create nearly any other cell type of the body. "Since all three in this protocol are major parts of vascular structure, it will be a useful tool to study a variety of diseases including eye disease," said Park.

Credit: NIH, National Eye Institute (NEI)

The five-step protocol, from iPSCs to fibroblasts, takes about four weeks. It includes seeding of iPSCs, seven days of monolayer differentiation, and cell separation based on CD31 expression. CD31 is a cell-surface protein unique to . At the end of step 3, endothelial and pericyte populations are separated. Fibroblasts can then be further differentiated from pericytes.

Park and colleagues are now working on a method to combine the three patient-derived cell types using bioprinting, which distributes and orients cells in a 3D space resembling the tissue microenvironment.

The paper is published in the journal STAR Protocols.

More information: Tea Soon Park et al, Protocol to generate endothelial cells, pericytes, and fibroblasts in one differentiation round from human-induced pluripotent stem cells, STAR Protocols (2023). DOI: 10.1016/j.xpro.2023.102292

Provided by NIH, National Eye Institute (NEI)
Citation: Scientists publish recipe for making blood vessel cells from patient stem cells (2023, May 16) retrieved 25 September 2023 from
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