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Subtyping of breast cancer cell lines reveals insights into cancer-relevant genes

Subtyping of breast cancer cell lines reveals insights into cancer-relevant genes
Waterfall plot depicting recurrent non-synonymous mutations in BC cell lines. Mutations were called on RNA-seq data for the gene set reported by Ciriello et al, in BRCA1 and BRCA2 and visualized as waterfall plot for the 36 genes in which mutations were identified. Credit: Cells (2024). DOI: 10.3390/cells13040301

Cell lines are an important in vitro model in breast cancer research. A team around biochemist Dr. Sonja Eberth and bioinformatician Dr. Claudia Pommerenke from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany) has extensively characterized the molecular properties of the breast cancer cell lines from the institute's collection.

The results of the study, recently published in Cells, allow the individual breast cancer to be assigned to the known clinically relevant breast cancer subtypes and provide insights into potentially new tumor-relevant genes. DSMZ is the first cell bank in the world to perform its own characterization of cancer cell lines using high-throughput sequencing, and to publicly present the and to make it available to the scientific community via DSMZCellDive.

Breast cancer comes in many different forms

Breast cancer is the most common cancer in women worldwide. However, breast cancer is not a uniform disease, as there are many different types. These subtypes exhibit different molecular alterations, for example in relation to the activity of hormone receptors, which are very important for diagnosis, treatment and prognosis.

To better understand these alterations in and establish new targeted approaches for therapy, in vitro models such as human cancer cell lines are indispensable in . These cell lines can grow almost indefinitely in a culture medium in the laboratory and be used for experiments.

However, for research results to be meaningful and interpretable, it is essential that a suitable model is used that can actually represent the tumor type under investigation with its molecular alterations.

The cell line collection of the DSMZ comprises more than 700 different human cancer cell lines for research purposes, including cell lines established from different primary or metastatic breast tumors.

Scientists from the DSMZ and the Hannover Medical School have now extensively studied and newly characterized the 29 breast cancer cell lines in the collection using various state-of-the-art methods. The molecular characteristics of the cell lines gained in this project will make it even easier for researchers worldwide to select suitable models for their breast cancer studies.

Free access to cell data via the DSMZ Digital Diversity platform

The data collected in the study is presented interactively on the open access web tool DSMZCellDive, a component of the DSMZ Digital Diversity Platform. Researchers can precisely check for each individual gene whether and in which breast cancer cell lines it is expressed.

This further optimizes the process of selecting suitable model cell lines for a specific research question. The cell lines with the appropriate characteristics can then be ordered from the DSMZ for research purposes.

More information: Claudia Pommerenke et al, Molecular Characterization and Subtyping of Breast Cancer Cell Lines Provide Novel Insights into Cancer Relevant Genes, Cells (2024). DOI: 10.3390/cells13040301

Provided by Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
Citation: Subtyping of breast cancer cell lines reveals insights into cancer-relevant genes (2024, March 19) retrieved 25 May 2024 from
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