Researchers connect neurons to computers to decipher the enigmatic code of neuronal circuits

Doctoral student Shein and his supervisors, Prof. Yael Hanein of the School of Electrical Engineering and Prof. Eshel Ben-Jacob of the School of Physics and Astronomy, want to understand the brain's logic. They have developed a new kind of a lab-on-a-chip platform that may help neuroscientists understand one of the deepest mysteries of our brain –– how neuronal networks communicate and work together. The chip was recently described in an issue of the journal PLoS ONE.

Within it, Shein has applied advanced mathematical and engineering techniques to connect neurons with electronics and understand how neuronal networks communicate. Hoping to answer ultimate questions about how our neuronal circuits work, the researchers believe their tool can be also used to test new drugs. It might also advance artificial intelligence and aid scientists in rewiring artificial limbs to our brain.

Shedding light on a black box

There are relatively simple neural "firing" patterns that can be measured with sensory organs like the ears or eyes, but researchers know little about deep thought processes. Could the brain's electrical signals reveal the basis of thought itself?

"When we look at the neuronal networks operating in the ears or eyes, we have some idea about the coding schemes they utilize," explains Shein. A researcher can apply a stimulus such as a bright light, for example, and then monitor responses in the eye's neurons. But for more complex processes, like "thinking" or operating different sensory inputs and outputs together, "we are basically looking into a black box," he says.

The brain is composed of a daunting number of circuits interconnected with other countless circuits, so understanding of how they function has been close to impossible. But using engineered brain tissue in a Petri dish, Shein's device allows researchers to see what's happening to well-defined neural circuits under different conditions. The result is an active circuitry of neurons on a man-made chip. With it they can look for patterns in bigger networks of neurons, to see if there are any basic elements for information coding.

Investigating the activity of single neurons is not enough to understand how a network functions. With nanotechnological systems and tools, now researchers can explore activity patterns of many neurons simultaneously. In particular, they can investigate how several groups of neurons communicate with each other, says Shein.

The hierarchy of the brain

With these network engineering techniques, the scientists cultured different sized networks of neuronal clusters. Once they looked at these groups, they found rich and surprising behaviors which could not be predicted from what scientists know about single neurons.

The researchers were also able to measure patterns from nerve activity, at nodes where a number of nerves converged into networks. What they detected appears to show that neural networks have a hierarchical structure — large networks are composed of smaller sub-networks. This observation, and a unique setup using electrodes and living nerves, allowed them to create hierarchical networks in a dish.

The brain's circuits work like codes. They can see the patterns in the networks and simplify them, or control connectivity between cells to see how the neuronal network responds to various chemicals and conditions, the scientists report. One theory, proposed by Prof. Ben-Jacob, is that the human brain stores memories like a holograph of an image: small neural networks contain information about the whole brain, but only at a very low resolution.

So far the researchers are able to reveal that clusters of as few as 40 cells can serve as a minimal but sufficient functional network. This cluster is capable of sustaining neural network activity and communicating with other clusters. What this means exactly will be the next question.

Provided by Tel Aviv University