Detecting circulating tumor cells

March 25, 2013

A proof-of-concept device is nearly perfect in separating breast cancer cells from blood.

About 1 in 4 deaths in the United States are due to cancer, but primary tumors are rarely fatal. Instead, it's when tumors metastasize that cancer becomes so deadly. To help patients and physicians make , teams of researchers have been working on various methods to detect cancer's spread – via the bloodstream – before secondary tumors develop. Now, one team reports a nearly perfect method for separating from blood. They describe their proof-of-concept device in a paper accepted for publication in Biomicrofluidics.

Detecting and separating (CTCs) is like finding the proverbial needle in a haystack: as few as one in a billion cells in a patient's bloodstream may be a CTC. Separation techniques vary widely, relying on differences in chemical, paramagnetic, or to distinguish CTCs from blood cells, or using mechanical sieves to cull the larger CTCs from the smaller blood cells. More recently, researchers have applied forces to fluid containing both and CTCs, using differences in inertia to sort cells. The technique, called "hydrodynamic sorting," is faster and easier than other sorting techniques. Like other mechanical techniques, it also allows researchers to collect viable cells after sorting them.

The team employed hydrodynamic sorting to develop their new device, called a multi-stage, multi-orifice flow fractionation (MS-MOFF) system. A previous design by the same team had just a single stage for applying hydrodynamic forces, but by adding an additional stage – so the output of the first stage becomes the input to the second stage – the researchers improved the separation efficiency of CTCs from 88.8% to 98.9%. Required pretreatment of the samples still makes MS-MOFF a proof-of-concept device, but the researchers suggest several ways to overcome such limitations and so make it useful for clinical applications.

Explore further: Circulating tumor cells not linked to survival in newly diagnosed inflammatory breast cancer

More information: "Continual collection and re-separation of circulating tumor cells (CTCs) from blood using multi-stage multi-orifice flow fractionation," is published in Biomicrofluidics.

Related Stories

Recommended for you

Combination therapy can prevent cytostatic resistance

November 26, 2015

Researchers at Karolinska Institutet have found a new way of preventing resistance to cytostatics used in the treatment of cancers such as medulloblastoma, the most common form of malignant brain tumour in children. The promising ...

Forecasting the path of breast cancer in a patient

November 23, 2015

USC researchers have developed a mathematical model to forecast metastatic breast cancer survival rates using techniques usually reserved for weather prediction, financial forecasting and surfing the Web.


Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.