News tagged with green fluorescent protein
(Medical Xpress)—People are living longer than ever before, thanks to medical and technological advances. Unfortunately, aging can be associated with a decrease in brain function. This is because, unlike ...
Neuroscience Feb 27, 2013 | 5 / 5 (5) | 0 |
In a ground-breaking study, researchers from the RIKEN Brain Science Institute in Japan report a new technique that allows them to visualize the distribution of retinoic acid in a live zebrafish embryo, in ...
Medical research Apr 07, 2013 | 4.3 / 5 (3) | 0 |
Scientists with the new Children's Research Institute at UT Southwestern Medical Center have identified the environment in which blood-forming stem cells survive and thrive within the body, an important step toward increasing ...
Medical research Jan 25, 2012 | 5 / 5 (1) | 0 |
(Medical Xpress)—If you want to read a mouse's mind, it takes some fluorescent protein and a tiny microscope implanted in the rodent's head.
Neuroscience Feb 19, 2013 | 4 / 5 (1) | 0 |
A team led by MIT neuroscientists has developed a way to monitor how brain cells coordinate with each other to control specific behaviors, such as initiating movement or detecting an odor.
Neuroscience Oct 17, 2012 | not rated yet | 0 |
Recent research has shown that there are new cells that develop in the heart, but how these cardiac cells are born and how frequently they are generated remains unclear. In new research from Brigham and Women's Hospital (BWH), ...
Cardiology Dec 05, 2012 | 5 / 5 (1) | 0 |
Inspired by earlier successes using gene therapy to correct an inherited type of blindness, investigators from the Perelman School of Medicine at the University of Pennsylvania, are poised to extend their ...
Medical research Jun 22, 2011 | not rated yet | 0 |
Stem cells are different from all other cells in our body because they retain the remarkable genetic plasticity to self-renew indefinitely as well as develop into cell types with more specialized functions. However, this ...
Cancer Apr 11, 2013 | 4 / 5 (1) | 0 |
Just as Gotham City uses the Bat Signal to call for Batman's aid, a new tool developed by scientists from the Sanford-Burnham Medical Research Institute in La Jolla, California, should serve as the cellular equivalent for ...
Medical research Jun 12, 2012 | not rated yet | 0
Chemicals in the environment that mimic estrogen can strongly influence the development of humans and other animals. New research to be presented at the 2012 International Zebrafish Development and Genetics Conference, held ...
Genetics Jun 21, 2012 | not rated yet | 0
Researchers from Mount Sinai School of Medicine have discovered the therapeutic benefit of fetal stem cells in helping the maternal heart recover after heart attack or other injury. The research, which marks a significant ...
Cardiology Nov 14, 2011 | not rated yet | 0 |
Fear conditioning using sound and taste aversion, as applied to mice, have revealed interesting information on the basis of memory allocation.
Neuroscience Jun 18, 2012 | 3 / 5 (1) | 0
Green fluorescent protein
The green fluorescent protein (GFP) is protein composed of 238 amino acids (26.9kDa), which exhibits bright green fluorescence when exposed to blue light. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm which is in the lower green portion of the visible spectrum. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. In modified forms it has been used to make biosensors, and many animals have been created that express GFP as a proof-of-concept that a gene can be expressed throughout a given organism. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation. To date, the GFP gene has been introduced and expressed in many bacteria, yeast and other fungi, fish (such as zebrafish), plant, fly, and mammalian cells, including human. Martin Chalfie, Osamu Shimomura, and Roger Y. Tsien were awarded the 2008 Nobel Prize in Chemistry on 8 October 2008 for their discovery and development of the green fluorescent protein.
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