Researchers invent device that makes chemotherapy more personalized, efficient

July 2, 2015 by Doug Bennett, University of Florida

Two University of Florida researchers have invented a device that makes chemotherapy treatments more personalized, efficient and affordable.

The miniaturized platform, known as a microarray, uses patients' cancer cells to test various doses and combinations of chemotherapy drugs. The device's breakthrough capability—its ability to work with a smaller number of —is especially crucial because such cells are particularly rare. Cancer stem cells comprise about 1 percent of a typical tumor, and other drug-testing methods require larger amounts of cells.

The device was developed by Benjamin G. Keselowsky, Ph.D., an associate professor in the J. Crayton Pruitt Family Department of Biomedical Engineering and Matthew R. Carstens, Ph.D., a post-doctoral associate in Keselowsky's laboratory. Researchers from the Cleveland Clinic and University of California collaborated on the research, which was published June 29 in the Proceedings of the National Academy of Sciences.

The microarray is a piece of square glass the size of a quarter that can hold a tiny grid of several hundred polymer "islands." The "islands" are first loaded with various combinations and doses of chemotherapy drugs. Cancer cells are then added, and the cells' interaction with the drugs is observed to determine which combinations work best, Carstens said.

The new array uses less than 6 percent of the cancer stem cells that are typically needed for such testing. Extracting elusive and rare cancer stem cells from patients is time consuming and expensive, and the new technique makes the process more efficient and cost-effective, Carstens said. The process has been granted a patent, although clinical trials may likely be required before it could be put to widespread use in clinics and laboratories, according to Keselowsky.

It also represents an advance in personalized medicine: A patient's cancer stem cells can be extracted and tested with various combinations of chemotherapy drugs. Currently, physicians try various drug combinations directly on patients—a process that the new microarray may someday replace.

"The potential for treatment is that we can do a lot of testing and know what will work in the patient. The ability to test multiple chemotherapy treatments with fewer is a big advancement," said Emina H. Huang, M.D., a colorectal surgeon at the Cleveland Clinic, professor of surgery at the Clinic's Lerner College of Medicine at Case Western Reserve University and vice chair of the Clinic's department of and regenerative medicine.

Some of the research was done in Huang's lab at UF Health, where she was a colorectal surgeon and professor of surgery. She also supplied the cells for the research and taught Carstens how to culture them.

While the microarray was tested on colorectal stem cells, Keselowsky said the approach could be used to test the efficacy of chemotherapy drugs on virtually any kind of cancer that involves a solid tumor. Chemotherapy for colorectal cancer is often a combination of two drugs, and researchers tested the interaction of the drugs nutlin-3a and camptothecin on cancer stem cells. The cells were taken from a 70-year-old, stage IV cancer patient and a 60-year-old patient with stage III colorectal cancer. The microarrays were seeded with approximately 200 cells per "island"—16 times fewer cells than other chemotherapy testing methods use.

The new array's efficient use of rare cells means that a patient could undergo a biopsy and the cancer stem cells could be tested with combinations of sooner. That would deliver the best treatment regimen to the patient in a timelier manner.

"Because it requires far fewer cells, there would be a quicker turnover time. That makes it possible to personalize the regimen much sooner," Keselowsky said.

In addition to potentially needing clinical trials, bringing the new microarray into widespread use would likely require a company to invest in further research and possibly production, Keselowsky said. Still, the invention is a significant advance in personalized medicine for cancer patients, he said.

"It's a combination of having a novel device that works on colon cancer , which is an important cancer biology topic right now," he said.

Explore further: Cancer uses stem cells as a shield to escape drug attacks

More information: Drug-eluting microarrays to identify effective chemotherapeutic combinations targeting patient-derived cancer stem cells, Matthew R. Carstens, DOI: 10.1073/pnas.1505374112

Related Stories

Cancer uses stem cells as a shield to escape drug attacks

June 27, 2014
Chemotherapy is one of the most important treatments for all types of cancer. It involves the use of drugs that kill abnormally multiplying cells. The therapy uses one or more drugs in combination and has been practised since ...

Scientists find way to disrupt brain tumor stem cells

June 11, 2015
Some brain tumors are notoriously difficult to treat. Whether surgically removed, zapped by radiation or infiltrated by chemotherapy drugs, they find a way to return.

Researchers identify new stem cell population important in the growth of colon cancer

June 16, 2015
Researchers at Lawson Health Research Institute have identified a new stem cell population in the colon linked to cancer growth. The findings, which were recently published in the prominent journal Cell Stem Cell, will significantly ...

Recommended for you

Research shows possible new target for immunotherapy for solid tumors

April 24, 2018
Research from the University of Cincinnati (UC) reveals a potential new target to help T cells (white blood cells) infiltrate certain solid tumors.

Changes in breast tissue increase cancer risk for older women

April 24, 2018
Researchers in Norway, Switzerland, and the United States have identified age-related differences in breast tissue that contribute to older women's increased risk of developing breast cancer. The findings, published April ...

Targeting molecules called miR-200s and ADAR2 could prevent tumor metastasis in patients with colorectal cancer

April 24, 2018
Colorectal cancer is the third most common cancer worldwide and the third-leading cause of cancer-related deaths. The main cause of death in patients with colorectal cancer is liver metastasis, with nearly 70% of patients ...

Removing the enablers: Reducing number of tumor-supporting cells to fight neuroblastoma

April 24, 2018
Investigators at the Children's Center for Cancer and Blood Diseases at Children's Hospital Los Angeles provide preclinical evidence that the presence of tumor-associated macrophages—a type of immune cell—can negatively ...

Technology used to map Mars now measuring effect of treatment on tumours

April 24, 2018
A machine learning approach for assessing images of the craters and dunes of Mars, which was developed at The University of Manchester, has now been adapted to help scientists measure the effects of treatments on tumours.

New test could tell doctors whether patients will respond to chemotherapy

April 24, 2018
Less than half the patients diagnosed with cancer respond favorably to chemotherapy, but a new method for testing how patients will respond to various drugs could pave the way for more personalized treatment.

0 comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.