Dormant viral genes may awaken to cause ALS

Dormant viral genes may awaken to cause ALS
Scientists studied human and mouse brains to show that genes for a seemingly inactive and inherited virus may cause ALS. Credit: Nath lab, NINDS, NIH, Bethesda, MD

Scientists at the National Institutes of Health discovered that reactivation of ancient viral genes embedded in the human genome may cause the destruction of neurons in some forms of amyotrophic lateral sclerosis (ALS). The results, published in Science Translational Medicine, suggest a link between human endogenous retroviral genes (HERVs) and ALS. The findings also raise the question of whether antiretroviral drugs, similar to those used for suppressing HIV, may help some ALS patients.

For generations, humans have been passing on genetic remnants of HERV infections that may have happened millions of years ago. Although nearly eight percent of the normal is made up of these , very little is known about their role in health and disease.

"People call the genes for these viruses junk DNA. Our results suggest they may become activated during ALS," said Avindra Nath, M.D., clinical director at the NIH's National Institute of Neurological Disorders and Stroke (NINDS) and a senior author of the study. "Ultimately we hope the results will lead to effective treatments for a heartbreaking disorder."

Currently, there is no effective treatment for the more than 12,000 Americans who live with ALS. This fatal disorder destroys that control movements, including speaking, walking, breathing and swallowing. On rare occasions, HIV-infected, AIDS patients develop ALS-like symptoms. In many of these patients, the symptoms can be reversed by treatment with . Previous studies found reverse transcriptase, a protein encoded by retroviral genes, in the blood of some ALS patients but its role in the disorder is unknown.

Avindra Nath, M.D., discusses results from a study he lead showing that HERV-K genes, a group of ancient, inherited retrovirus genes may awaken to cause some forms of ALS. Credit: NINDS

These observations prompted Dr. Nath and his team to explore the possible link between retroviruses and ALS. Unexpectedly they found that endogenous, or inherited, retroviruses may be involved with ALS.

Initially, they showed that brain samples from ALS patients had higher than normal levels of messenger RNA (mRNA) encoded by genes of the human endogenous retrovirus K (HERV-K). A protein encoded by a critical HERV-K gene, called env, was found in brain samples from ALS patients but not from healthy individuals or patients with Alzheimer's disease. They also showed that activation of HERV-K genes killed healthy human neurons grown in petri dishes.

To test the role of HERVs in ALS, the scientists genetically modified mice so that their neurons activated the HERV-K env gene. The mice died earlier than normal and had problems with balance and walking that progressively worsened with age. When the scientists inspected the brains, spinal cords and muscles of these mice they found that only motor neurons, the cells that control movements and die in ALS, were damaged. Cells in other parts of the nervous system remained healthy.

"We showed that may be susceptible to activation of these genes during ALS," said Dr. Nath.

Finally the scientists showed that activation of HERV-K genes may be controlled by TDP-43, a gene-regulating protein that has been strongly linked to ALS and known to control HIV production. Genetically enhancing TDP-43 in human neurons increased the cells' production of HERV-K mRNA and proteins whereas genetically blocking TDP-43 in other cells reduced HERV-K reverse transcriptase activity.

Dr. Nath and his team are now collaborating with the ALS center at Johns Hopkins University to study whether antiretroviral treatments are effective at controlling HERV-K replication in a subset of patients with ALS.

"We may have discovered a precision medicine solution for treating a neurodegenerative disorder," said Dr. Nath.

More information: Li et al. "Human endogenous retrovirus-K induces motor neuron disease," Science Translational Medicine, September 30, 2015. DOI: 10.1126/scitranslmed.aac8201
Journal information: Science Translational Medicine

Citation: Dormant viral genes may awaken to cause ALS (2015, September 30) retrieved 30 November 2022 from
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