Increasing the activity of the neurotransmitter GABA in the brains of depressed mice has antidepressant effects
Experimentally increasing the activity of a subclass of nerve cells that produce the neurotransmitter gamma-aminobutyric acid (GABA) has antidepressant effects similar to pharmaceutical antidepressants in depressed mice. The discovery lends new credence to the idea that GABA-enhancing drugs could serve as rapidly acting and more effective antidepressants. A paper describing the research, led by Penn State scientists, appears online in the journal Molecular Psychiatry on November 8, 2016.
"GABA is the major inhibitory neurotransmitter in the brain—it acts as the "brakes" of neural activity—and its dysfunction is implicated in a wide range of neuropsychiatric disorders," said Bernhard Lüscher, professor of biology and of biochemistry and molecular biology at Penn State and the leader of the research team. "Increasing evidence suggests that dysfunction of GABA is also a major culprit in major depressive disorder, the most common and costly brain disorder and a principal cause of suicide, the primary cause of death among young people."
The researchers increased GABA signaling by disabling a GABA receptor in a specific set of neurons that are suspected to be involved in major depressive disorder. Under normal circumstances, this set of neurons known as SST+ interneurons (somatostatin-positive-GABAergic interneurons) produce GABA, which reduces the activity of other neurons around them. Most of these surrounding neurons release the neurotransmitter, glutamate—an "accelerator" of neuron activity. When the researchers disabled the GABA receptor selectively in SST+ interneurons, these cells could no longer receive the signal to slow down—their "brakes" were removed—so they released GABA excessively, which in turn slowed even further the activity of glutamate-producing neurons. As a result, the mice who received this treatment acted in a number of behavioral tests as if they had been given antidepressant drugs. Moreover, biochemical changes in brain tissue of the genetically altered mice mimicked biochemical changes previously observed in rodents subjected to pharmacological treatment with different classes of antidepressant drugs.
Currently used antidepressants are designed to enhance the function of serotonin, or less often, norepinephrine. These drugs are ineffective for about 40 percent of patients and they suffer from a characteristically slow therapeutic onset, taking weeks before patients notice any significant improvement. This slow mode of therapeutic action indicates that the mechanism of these drugs is only distantly related to their direct targets. Instead, the current work suggests that these drugs ultimately act by enhancing the function of GABA-releasing interneurons. Indeed, earlier research in the Lüscher lab has shown that these drugs can alleviate a depressive-like brain state in mice that was induced by genetic defects in GABA transmission. Thus, antidepressants that act through serotonin or norepinephrine ultimately appear to normalize defects in GABA transmission, suggesting that new drugs that target GABA signaling may be effective antidepressants.
"The GABA-producing neurons we manipulated in this research are characterized by expression of a neuropeptide known as somatostatin and they represent about 30 percent of all GABA-producing cells in the forebrain," said Lüscher. "These cells have recently been implicated in major depression based on gene-expression changes found in postmortem brain tissue from depressed patients, and they differ from other GABA-producing cells in that they selectively innervate the dendritic trees of glutamate-releasing neurons. The somatostatin-positive subset of GABA-releasing cells therefore appear ideally suited to limit the overexcitation of neural networks that occurs during the chronically stressful situations that figure as major culprits in depressive disorders."
The results of the research lend new credence to the idea that GABA-enhancing drugs could serve as effective, rapidly acting antidepressants. However, it appears to be essential that these drugs selectively enhance the activity of the somatostatin-positive subset of GABA-producing cells or the subset of GABA receptors that mediate the response to GABA signaling originating from these cells.