Circulating exosomes have distinct RNA profile in MS

Circulating exosomes have distinct RNA profile in MS

(HealthDay)—Circulating exosomes have a distinct RNA profile in relapsing-remitting multiple sclerosis (RRMS), according to a study published online April 15 in the Annals of Neurology.

Igor Selmaj, M.D., from the Medical University of Lodz in Poland, and colleagues used next generation sequencing to define the global RNA profile of serum exosomes in 19 RRMS patients and 10 healthy controls (HCs). Per sample, they analyzed 5,000,000 reads and over 50,000 transcripts, including a detailed analysis of microRNA (miRNA) differentially expressed in RRMS. The discovery data set was validated using data from an independent cohort of 63 RRMS patients (33 in relapse and 30 in remission) and 32 HCs.

The researchers found that in RRMS patients versus HCs, 15 different classes of transcripts detected four circulating exosomal sequences within the miRNA category that were differentially expressed: hsa-miR-122-5p, hsa-miR-196b-5p, hsa-miR-301a-3p, and hsa-miR-532-5p. During relapse there was a significant decrease of serum exosomal expression of these miRNA in RRMS. In with a gadolinium enhancement on brain magnetic resonance imaging, these miRNAs were also decreased. In RRMS, in vitro secretion of these miRNAs by peripheral blood mononuclear cells was significantly impaired.

"Since putative targets for these miRNA include the signal transducer and activator of transcription 3 and the cell cycle regulator aryl hydrocarbon receptor the data suggest a disturbed cell-to-cell communication in this disease," the authors write.

One author was supported by a grant from Genzyme.

More information: Abstract
Full Text (subscription or payment may be required)

Journal information: Annals of Neurology

Copyright © 2017 HealthDay. All rights reserved.

Citation: Circulating exosomes have distinct RNA profile in MS (2017, April 20) retrieved 9 June 2023 from
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.

Explore further

Researchers link body temperature to relapsing-remitting MS and fatigue


Feedback to editors