Protein prevents excess fluid from entering lung tissue

Lung tissue. Credit: Rutgers University

A protein found in the cells lining blood vessels plays a central role in preventing fluid and inflammatory cells from leaking into lung tissue in a low-oxygen environment, Weill Cornell Medicine researchers discovered.

Their findings may enable the development of more effective treatments for pulmonary edema, in which excess fluid makes it difficult to breathe.

In the study, published July 10 in the Journal of Experimental Medicine, the scientists genetically engineered mice without the annexin A2. They exposed rodents with and without the protein to a low-oxygen or normal-oxygen environment for 48 hours, injected an experimental dye and then examined their organs. The investigators found that mice without annexin A2 showed a two-fold greater leakage of dye into compared to mice with the protein.

Annexin A2 is one of the proteins found in the junctions between tightly packed endothelial , which line . The junctions function like gates keeping fluid out. When oxygen is low in the lung, compounds called phosphates can stick onto the gates, jamming them open and allowing fluid and to pass into tissue. The researchers discovered that annexin A2 links with another protein called vascular endothelial cadherin (VEC) and two enzymes, endothelial-specific and Src homology phosphatase-2, that help remove the phosphates and keep the gates closed.

"Our novel finding, that annexin A2 enables the enzyme activity that prevents vascular leak in mice, suggests that it may play a similar role in humans," said senior study author Dr. Katherine A. Hajjar, a professor of pediatrics, the Brine Family Professor of Cell and Developmental Biology and professor of pediatrics in medicine at Weill Cornell Medicine.

Hajjar and colleagues, including Min (Lucy) Luo, senior research associate of cell and developmental biology in pediatrics at Weill Cornell Medicine, found that annexin A2 played a gatekeeper role only in lung, but not in the brain or other organs. "We were surprised to find this effect," Hajjar said. "Further research is needed to determine why, but it may be because lung endothelial cells are very thin and adapted for air exchange."

This new insight sets the stage for future research into annexin A2 in humans and its effect on pulmonary edema, which can occur when the body is deprived of oxygen or in sepsis.

"It is conceivable that babies born with asphyxia or adults with severe sepsis might be predisposed to if they also have insufficient annexin A2 or genetically based variants," she said.

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More information: Min Luo et al. Annexin A2 supports pulmonary microvascular integrity by linking vascular endothelial cadherin and protein tyrosine phosphatases, The Journal of Experimental Medicine (2017). DOI: 10.1084/jem.20160652
Journal information: Journal of Experimental Medicine

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Citation: Protein prevents excess fluid from entering lung tissue (2017, July 18) retrieved 25 January 2021 from
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