Using optogenetics and holographic projection, scientists aim to implant perceptions in brain

Editing brain activity with holography
Sample hologram with 50 randomly distributed neuron targets spanning a region 500 microns square and 250 microns deep. Credit: Adesnik lab, UC Berkeley

What if we could edit the sensations we feel; paste in our brain pictures that we never saw, cut out unwanted pain or insert non-existent scents into memory?

University of California, Berkeley neuroscientists are building the equipment to do just that, using holographic projection into the to activate or suppress dozens and ultimately thousands of at once, hundreds of times each second, copying real patterns of to fool the brain into thinking it has felt, seen or sensed something.

The goal is to read neural activity constantly and decide, based on the activity, which sets of neurons to activate to simulate the pattern and rhythm of an actual brain response, so as to replace lost sensations after , for example, or control a prosthetic limb.

"This has great potential for neural prostheses, since it has the precision needed for the brain to interpret the pattern of activation. If you can read and write the language of the brain, you can speak to it in its own language and it can interpret the message much better," said Alan Mardinly, a postdoctoral fellow in the UC Berkeley lab of Hillel Adesnik, an assistant professor of molecular and cell biology. "This is one of the first steps in a long road to develop a technology that could be a virtual brain implant with additional senses or enhanced senses."

Mardinly is one of three first authors of a paper appearing online April 30 in advance of publication in the journal Nature Neuroscience that describes the holographic brain modulator, which can activate up to 50 neurons at once in a three-dimensional chunk of brain containing several thousand neurons, and repeat that up to 300 times a second with different sets of 50 neurons.

"The ability to talk to the brain has the incredible potential to help compensate for neurological damage caused by degenerative diseases or injury," said Ehud Isacoff, a UC Berkeley professor of molecular and and director of the Helen Wills Neuroscience Institute, who was not involved in the research project. "By encoding perceptions into the human cortex, you could allow the blind to see or the paralyzed to feel touch."

Holographic projection

Each of the 2,000 to 3,000 neurons in the chunk of brain was outfitted with a protein that, when hit by a flash of light, turns the cell on to create a brief spike of activity. One of the key breakthroughs was finding a way to target each cell individually without hitting all at once.

To focus the light onto just the cell body - a target smaller than the width of a human hair - of nearly all cells in a chunk of brain, they turned to computer generated holography, a method of bending and focusing light to form a three-dimensional spatial pattern. The effect is as if a 3D image were floating in space.

In this case, the holographic image was projected into a thin layer of brain tissue at the surface of the cortex, about a tenth of a millimeter thick, though a clear window into the brain.

"The major advance is the ability to control neurons precisely in space and time," said postdoc Nicolas Pégard, another first author who works both in Adesnik's lab and the lab of co-author Laura Waller, an associate professor of electrical engineering and computer sciences. "In other words, to shoot the very specific sets of neurons you want to activate and do it at the characteristic scale and the speed at which they normally work."

The researchers have already tested the prototype in the touch, vision and motor areas of the brains of mice as they walk on a treadmill with their heads immobilized. While they have not noted any behavior changes in the mice when their brain is stimulated, Mardinly said that their brain activity - which is measured in real-time with two-photon imaging of calcium levels in the neurons - shows patterns similar to a response to a sensory stimulus. They're now training mice so they can detect behavior changes after stimulation.

Prosthetics and brain implants

The area of the brain covered - now a slice one-half millimeter square and one-tenth of a millimeter thick - can be scaled up to read from and write to more neurons in the brain's outer layer, or cortex, Pégard said. And the laser holography setup could eventually be miniaturized to fit in a backpack a person could haul around.

Mardinly, Pégard and the other first author, postdoc Ian Oldenburg, constructed the holographic brain modulator by making technological advances in a number of areas. Mardinly and Oldenburg, together with Savitha Sridharan, a research associate in the lab, developed better optogenetic switches to insert into cells to turn them on and off. The switches - light activated ion channels on the cell surface that open briefly when triggered - turn on strongly and then quickly shut off, all in about 3 milliseconds, so they're ready to be re-stimulated up to 50 or more times per second, consistent with normal firing rates in the cortex.

Pégard developed the system using a liquid crystal screen that acts like a holographic negative to sculpt the light from 40W lasers into the desired 3D pattern. The lasers are pulsed in 300 femtosecond-long bursts every microsecond. He, Mardinly, Oldenburg and their colleagues published a paper last year describing the device, which they call 3D-SHOT, for three-dimensional scanless holographic optogenetics with temporal focusing.

"This is the culmination of technologies that researchers have been working on for a while, but have been impossible to put together," Mardinly said. "We solved numerous technical problems at the same time to bring it all together and finally realize the potential of this technology."

As they improve their technology, they plan to start capturing real patterns of activity in the cortex in order to learn how to reproduce sensations and perceptions to play back through their holographic system.

Explore further

Revolutionary brain-mapping technique provides new blueprint for cortical connections

More information: Alan R. Mardinly et al, Precise multimodal optical control of neural ensemble activity, Nature Neuroscience (2018). DOI: 10.1038/s41593-018-0139-8
Journal information: Nature Neuroscience

Citation: Using optogenetics and holographic projection, scientists aim to implant perceptions in brain (2018, April 30) retrieved 19 July 2019 from
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Apr 30, 2018
What if we could edit the sensations we feel; paste in our brain pictures that we never saw, . . . or insert non-existent scents into memory?

Can anybody think of any possible way technology like this could be abused? Has anybody seem a movie that explored this topic?

Apr 30, 2018
"No problem is so serious, that all out Human effort cannot help but make it worse!"

"Knowledge is not Education.
Education is not Experience.
Experience is not Expertise.
Expertise is not Wisdom.

Ut has been my life experience that it takes the smartest of people to commit the most stupid of mistakes.

Apr 30, 2018
This is old tech. The CIA uses it to cause people to commit suicide.

Apr 30, 2018
This is old tech. The CIA uses it to cause people to commit suicide.

and evidently some people to have their brains explode...

May 01, 2018
You ask "Can anybody think of any possible way technology like this could be abused? Has anybody seem a movie that explored this topic?"

Certainly there are MANY TV shows and movies that have explored this topic. For example; (a) STAR TREK T.O.S. episodes "Dagger of the Mind" ( and "The Return of the Archons" (; (b) STARGATE SG-1 episode "Rervisions" (; (c) and loads more.

Plus, as you already may be know, news-reports are NOW SAYING that China's government has OFFICIALLY put into operation an AI-based system of READING WORKERS' (and citizens') MINDS, with the ostensible goal of REDUCING TENSIONS (?) and of MAKING THE WORKERS MORE EFFICIENT AT THEIR TASKS (

Or perhaps your question was rhetorical?

May 01, 2018
Protonius, I was trying to provoke a response. How this technology news could be reported without the slightest hint of doubt about its misuse is beyond me. It seems like a ton of movies and television shows have touched on this. For example, the Matrix Trilogy relied on implanting images. BTW, Star Trek TNG also had a number of episodes like this. In one the Romulans brainwashed Chief Engineer Geordi La Forge.

May 01, 2018
MT i just fail too see the relevance in encouraging the comicbook influenced "True Believers". They already poison social discourse conflating fictional speculations with real life.

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