Osteoarthritis assessment to go hi-tech

Osteoarthritis assessment to go hi-tech
Examining joint cartilage. Credit: ARC Centre of Excellence for Nanoscale BioPhotonics

Scientists have reported an advanced imaging technique that allows the condition of joint cartilage to be examined—right down to the molecular level. The technique has potential for diagnostics and treatment-planning of cartilage disease and impairment, including for osteoarthritis.

"Damage and degradation of cartilage around joints leads to severe pain and loss of mobility," says Dr Saabah Mahbub, Research Fellow at the ARC Centre of Excellence for Nanoscale BioPhotonics (CNBP) and lead author of the published study.

"We need a tool to help us to determine objectively, the degree of problem that the is exhibiting. We then need a way to be able to monitor the effectiveness of any cartilage regeneration therapies that are able to be undertaken," he says.

"Ideally we need to be able to do this monitoring at a and in a minimally invasive way."

A cutting-edge technique termed was used by Dr Mahbub to achieve this. This combined the power of an advanced optical microscope together with high powered , to measure and image the electromagnetic light-waves being given off by the cartilage tissue and cartilage cells known as chondrocytes.

"In this study, we applied our advanced hyperspectral microscopy to osteoarthritic human cartilage—to investigate its capacity to generate molecular data and to help us characterise the cartilage disease-state, as well as to examine potential treatment effects," he says.

"Using this approach, we were able to identify types and amounts of (collagen I and collagen II) in the cartilage tissue as well as to test for the specific co-enzymes FAD and NADH in the ."

In a breakthrough for Dr Mahbub, the hyperspectral-based study was also capable of detecting effects related to cartilage treatments (in this case the use of secretions from stem cells). This was indicated by hyperspectral imagery indicating changes in the composition of the cartilage – ratios of collagen I to collagen II – when comparing pre and post cartilage treatment activity."

"We believe that levels of collagen I, collagen II and associated proteins may be sensitive to improvements in cartilage health and could be used to monitor patient progression and to discern between effects of different osteoarthritis therapies," says Dr Mahbub.

Osteoarthritis assessment to go hi-tech
Dr Saabah Mahbub, CNBP Credit: Tony Crawshaw

It is envisaged that, in the future, this new imaging technique will be available to patients in a clinical setting where it would be deployed endoscopically through small incisions in targeted areas of the body.

Next steps for Dr Mahbub and the research team are to further investigate the molecular underpinnings of the progression of deterioration as well as regeneration.

This research was reported in the journal Scientific Reports with scientists affiliated with CNBP, Macquarie University, UNSW Sydney, Quantitative Pty Ltd and Regeneus Pty Ltd. Dr Saabah Mahbub was a CNBP Research Fellow based at Macquarie University when the research was undertaken.


Explore further

Scientists uncover why knee joint injury leads to osteoarthritis

More information: Saabah B. Mahbub et al. Non-Invasive Monitoring of Functional State of Articular Cartilage Tissue with Label-Free Unsupervised Hyperspectral Imaging, Scientific Reports (2019). DOI: 10.1038/s41598-019-40942-7
Journal information: Scientific Reports

Citation: Osteoarthritis assessment to go hi-tech (2019, March 14) retrieved 19 April 2019 from https://medicalxpress.com/news/2019-03-osteoarthritis-hi-tech.html
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.
17 shares

Feedback to editors

User comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more