Hole-forming protein may suppress tumor growth

Hole-forming protein may suppress tumor growth
GSDME regulates caspase-3 activation and pyroptosis in CEM-C7 cells. a Immunoblots of GSDME, caspase-3, and β-actin in cell lysates (Lys), or high-mobility group box 1 (HMGB1) released in culture media (media) of CEM-C7 cells untreated (UT) or triamcinolone acetonide (TA) treated for the indicated times. b Cytotoxicity of TA as measured by lactate dehydrogenase (LDH) release in the culture supernatants of untreated (Untreated) or TA-treated (TA) CEM-C7 cells for the indicated times. c, d Propidium iodide (PI) uptake (c), and active caspase-3 staining (d–g) in wild-type (WT) and GSDME-knockout (KO) CEM-C7 cells treated with TA (d), etoposide (e), ultraviolet (UV) (f), or serum starvation (g) as measured on the IncuCyte over time. h Immunoblots of GSDME, caspase-3, and β-actin in combined cell lysates plus culture media  of WT and GSDME-KO (KO) CEM-C7 cells untreated (UT) or treated with TA, etoposide, serum starvation, or UV as indicated. Results are representative of at least three independent experiments performed in duplicate or triplicate. Error bars represent standard deviation (S.D.). Student’s t-test, *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.00005, ******p < 0.0000005. Credit: Nature Communications (2019). DOI: 10.1038/s41467-019-09397-2

Sometimes cells need to die. The process of cell death is encoded within the genome of all higher organisms to kill off cancerous cells, and as a normal part of development to shape a mass of embryonic cells into the organism it will become. Now Jefferson researchers show a gene called gasdermin E, which is downregulated in many cancers, aids cells in dying in an unexpected way, and may also suppress tumor growth.

"We found that gasdermin E might be important in controlling growth," says Emad Alnemri, Ph.D., the Thomas Eakins endowed Professor of Biochemistry and Molecular Biology at Sidney Kimmel Cancer Center—Jefferson Health (SKCC), who led the new research.

The researchers published the findings April 11th in the journal Nature Communications.

In many breast, gastric and , gasdermin E gene expression is much lower than in healthy cells. Why cancers might turn down the expression of this gene was unknown. In previous research, Dr. Alnemri and colleagues found that gasdermin E participates in the cell death program when a cell death enzyme called caspase-3 cleaves it. The researchers discovered that the cleaved gasdermin E creates holes in the outer membrane of the cell. The holes cause the cell to swell and burst. The scientists thought a similar mechanism could be at work in .

When cells die, they undergo a series of coordinated, pre-programmed steps to self-destruct in a process known as apoptosis. When Dr. Alnemri and team treated cancer cells with a chemotherapy drug that sets the death cascade in motion, they found gasdermin E advances apoptosis by forming holes—not only in the cell membrane, but also in the membrane of organelles essential for life, the mitochondria, the cell's powerhouse. When gasdermin E pokes holes in mitochondria, it unleashes the proteins that carry out the final phases of programmed cell death.

Before gasdermin E can act, caspase-3 must clip off a piece of gasdermin E for the protein to be able to make holes in membranes. Likewise, caspase-3 must also be activated to carry out its job. When Dr. Alnemri and colleagues removed gasdermin E expression from cells, they found activation of caspase-3 was considerably lower. The finding means gasdermin E also acts as another "on" switch for caspase-3. The discovery suggests gasdermin E and caspase-3 engage in a feed-forward loop that accelerates apoptosis, which could help control cancer growth.

To find out if gasdermin E might control cancer growth, the researchers deleted the gene from three cancer cell lines. They found that without gasdermin E, the cancer cells multiplied about twice as fast. The researchers went on to show melanoma cells lack gasdermin E form and grow larger tumors in mice. In contrast, when cancer cells retain gasdermin E expression, they have slower growth.

"We found that cells with gasdermin E grow less and are actually more susceptible to death," says Dr. Alnemri. "They don't form tumors as well either, while the that lack this protein form bigger tumors and kill the mice much faster."

The finding suggests gasdermin E limits cancer growth and progression and may serve as a way to tell whether a is fast growing.

"Gasdermin E could be used as a marker to distinguish aggressive tumors from less aggressive ones," says Dr. Alnemri. "Aggressive tumors may have no gasdermin E, but less aggressive ones could have more." Likewise, the information could be used to decide how to design effective strategies to treat cancer.

The findings also suggest gasdermin E may be a cancer therapy target. Many tumors silence the expression of gasdermin E, but chemotherapies such as decitabine that reactivate its expression may be helpful.

Still, which cancers can be kept in check by gasdermin E expression, is an open question, Dr. Alnemri cautions. "Gasdermin E might control and work as a biomarker in one type of cancer, but not another," he says.

Explore further

Cell death: How a protein drives immune cells to suicide

More information: Corey Rogers et al, Gasdermin pores permeabilize mitochondria to augment caspase-3 activation during apoptosis and inflammasome activation, Nature Communications (2019). DOI: 10.1038/s41467-019-09397-2
Journal information: Nature Communications

Citation: Hole-forming protein may suppress tumor growth (2019, April 22) retrieved 24 November 2020 from https://medicalxpress.com/news/2019-04-hole-forming-protein-suppress-tumor-growth.html
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