New discovery on the activity and function of MAIT cells during acute HIV infection

New discovery on the activity and function of MAIT cells during acute HIV infection
RNA-Seq was performed on sorted MAIT cells from the PBMC of longitudinal samples corresponding to one pre-infection and three post-infection time points in the acute capture cohort (n = 9). a Volcano plots depict upregulated (red) or downregulated (blue) genes compared to pre-infection at three post-infection time points in acute HIV-1 infection. Individual genes listed in Supplementary Table 4. Highlighted genes have a –Log10 p-value ≥ 3 and a Log2 fold change of 0.5 or −0.5 (corresponding to p ≤ 0.001, and fold change of 1 or −1, in a generalized linear model). b The temporal dynamics of the upregulated and downregulated genes shown longitudinally in acute HIV-1 infection, together with plasma VL. c Shared and unshared differently expressed genes compared to pre-infection between all three post-infection time points in acute HIV infection are highlighted as a Venn diagram, and listed in Supplementary Table 4. d Gene expression patterns were subjected to Gene Set Enrichment Analysis (GSEA), and upregulated and downregulated pathways in post-infection time points compared to pre-infection are displayed as a Normalized Enrichment Score (NES) heat map. Enrichment plots from three selected post-infection upregulated pathways compared to pre-infection are shown; e, negative regulation of viral entry into host cell, f, regulation of IFNγ production, and g, natural killer cell mediated immunity. Genes contributing to enrichment plots are listed in Supplementary Table 5. PBMC, Peripheral blood mononuclear cells. VL, viral load. MAIT cells are identified as CD161 + + Vα7.2 + cells within CD3 + CD14-CD19- live lymphocytes. Credit: Nature Communications

In a new study published in Nature Communications, researchers at Karolinska Institutet show that mucosa-associated invariant T cells (MAIT cells) of the human immune system respond with dynamic activity and reprogramming of gene expression during the initial phase of HIV infection. The study fills a knowledge gap, as previously, the function of MAIT cells during this particular phase was not well understood.

Major efforts have been made in recent years to understand how people's immune systems react and act during the first days and weeks of HIV infection.

"Through a close collaboration with the Walter Reed Army Institutes of Research, we have gained access to an extensive biobank where we have been able to study samples from individuals, both before and after they have been infected with HIV," says Johan Sandberg, professor at the Department of Medicine at Karolinska Institutet in Huddinge and senior author of the study.

MAIT cells are part of the immune system. Their primary task is to control bacteria at the body's barriers, such as skin and mucous membranes. Among other things, MAIT cells have an important function in combating bacterial infections of the lung, for example, tuberculosis. However, MAIT cells also respond during an HIV infection, even though it is a viral infection. This is probably because the immune systems of infected individuals lose control over bacteria when infected.

"Previous observations have shown that MAIT cells disappear in the later stages of HIV infection. In this study, we can see that the MAIT cells actually expand at the initial stage with a strong activation in order to fight the bacteria," says Johan Sandberg.

MAIT cells may not help to control the HIV virus to any great extent, but when the infection becomes chronic, the number of MAIT cells in the body decreases. Unlike many other parts of the body's immune system, MAIT cells do not recover when the HIV infection is treated.

"In the study, we also discovered that the gene expression patterns of the MAIT cells change gradually during the initial phase," says Johan Sandberg. "They take on different characteristics and their antibacterial function declines, which can affect the individual's immune defence in a negative way."

The study increases the understanding of what happens at the initial phase of HIV infection. Knowledge has previously been lacking in this area, as the individuals diagnosed with HIV had often been infected for a longer time when the research began.

"In the HIV research that is currently being conducted, the focus is on developing vaccines and finding cures," says Johan Sandberg. "Knowledge of how the immune system works in the initial phase of HIV , such as the activity and properties of the MAIT , can facilitate the finding of future interventions."

More information: Dynamic MAIT cell response with progressively enhanced innate-like features during acute HIV-1 infection, Nature Communications, Jan. 14, 2020, DOI: 10.1038/s41467-019-13975-9

Journal information: Nature Communications
Citation: New discovery on the activity and function of MAIT cells during acute HIV infection (2020, January 14) retrieved 12 June 2024 from
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