A step closer towards a more effective treatment for osteoarthritis

A step closer towards a more effective treatment for osteoarthritis
In vivo images of mice knee joints obtained from Bruker© Xtreme II on day 7 after intra-articular injection of (a) Cy7 free dye and (b) Cy7-labeled PLGA Microparticles Credit: Kaamini M Dhanabalan

Researchers at the Indian Institute of Science (IISc) have developed a microparticle formulation that allows sustained release of drugs to treat osteoarthritis, a chronic joint condition.

They have designed a polymer matrix made of poly (lactic-co-glycolic acid) or PLGA, an FDA-approved biomaterial, to encapsulate rapamycin, an immunosuppressant drug. Preliminary studies on cells cultured in the laboratory as well as in mice models have shown promising results indicating reduced inflammation and due to sustained drug release.

"In cell studies, rapamycin-loaded PLGA microparticles could release the drug for up to 21 days, and in animal studies, PLGA microparticles showed up to 30 days after injecting the microparticles in the mice knee joint," says Kaamini M Dhanabalan, Ph.D. student at the Center for BioSystems Science and Engineering (BSSE), IISc, and first author of the study published in the journal Biomaterials Science.

Osteoarthritis is associated with the wear and tear of the cartilage ‒ the smooth tissue that protects bone joints ‒ caused due to stress or aging. The current treatment plan revolves more around managing pain and inflammation than treating the disease. Although several classes of drugs seemed promising in preclinical trials, low drug retention and rapid clearance from the target site have made clinical translation difficult.

The formulation developed by the IISc team, however, has a residence time of up to 30 days at the target site, with no evident signs that it may cause discomfort to patients. Such a sustained release system can improve patient compliance and reduce hospital visits.

PLGA is widely used for drug delivery applications and several drug formulations are currently used in clinics. Rapamycin is commonly used to suppress immune response in patients undergoing surgery for organ transplant to prevent organ rejection. Preclinical studies have shown its potential for treating by preventing cell loss and cartilage damage, thereby reducing inflammation. However, the short drug retention time of ~1-4 hours demands frequent injections to maintain the therapeutic window in the joints. Therefore, Dhanabalan and her colleagues combined the advantages of PLGA and rapamycin to create a system that would allow sustained release of the . This was achieved by encapsulating rapamycin in PLGA microparticles.

To evaluate the effectiveness of this formulation, chondrocytes or cartilage cells were cultured and subjected to various stresses to recreate osteoarthritis-like conditions under laboratory settings. This resulted in ailing chondrocytes with the hallmarks of osteoarthritis. These damaged chondrocytes recovered from osteoarthritis when treated with rapamycin-loaded PLGA microparticles.

"Preliminary studies using this newly-designed formulation can potentially reduce the frequent medical intervention to once a month. Detailed studies are in progress to explore the functional potential in mouse models of osteoarthritis," says Rachit Agarwal, Assistant Professor at BSSE, IISc, and senior author of the study.


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More information: Kaamini M. Dhanabalan et al. Rapamycin–PLGA microparticles prevent senescence, sustain cartilage matrix production under stress and exhibit prolonged retention in mouse joints, Biomaterials Science (2020). DOI: 10.1039/d0bm00596g
Journal information: Biomaterials Science

Citation: A step closer towards a more effective treatment for osteoarthritis (2020, July 6) retrieved 12 August 2020 from https://medicalxpress.com/news/2020-07-closer-effective-treatment-osteoarthritis.html
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