Lateral flow testing should not be used as a green light for activities

covid swab
Credit: Unsplash/CC0 Public Domain

The United Kingdom government plans to implement mass scale population testing for SARS-CoV-2 infection using Lateral Flow Devices (LFDs), yet the devices' sensitivity is unknown. A study published in the open access journal PLOS Biology by Alan McNally at University of Birmingham, UK, and colleagues suggests while LFDs are highly effective in identifying SARS-CoV-2 in individuals with high quantities of viral RNA present on the test swab, they are inaccurate at diagnosing infections in individuals with lower viral loads.

LFDs are increasingly used to increase testing capacity and screen asymptomatic populations for SARS-CoV-2 in mass surveillance programs, yet there are few data indicating the performance of these devices. To determine the sensitivity of LFDs, researchers tested the nasopharyngeal swabs of 8,000 using the devices. Next, they validated all positive samples and randomly selected 720 negative samples out of 7,187 negatives for validation.

Using reverse transcription PCR tests, the authors estimated the true COVID-19 prevalence rate and compared it to LFD test results. The researchers were able to determine the LFDs' accuracy rate for different levels of virus, finding that while the test was able to detect infections in samples with higher levels of viral RNA at a 100% accuracy rate, the test's performance dropped significantly in samples with lower viral loads.

The results suggest that for individuals in the very early or very late stages of infection, LFDs are likely to produce a false negative result, pointing to the need for frequent testing. A limitation of the study was that emergent variants and their potential effects on LFDs' sensitivity with asymptomatic cases were not considered within the scope of the study. Independent research suggests that both the UK and South African variants can be reliably detected, but further studies are needed to determine whether the LFDs can detect infections at the same viral loads as with the SARS-CoV-2 strain examined in the current research.

According to the authors, "When used regularly, LFDs can be a highly effective tool in reducing overall community burden. We would strongly recommend that Lateral Flow Device testing is used to screen people at a very regular frequency, and that a negative result should not be used to determine that someone is free from SARS-CoV-2 infection. As such LFDs should not be used as a -to-do."


Explore further

Follow the latest news on the coronavirus (COVID-19) outbreak

More information: Jack Ferguson et al. Validation testing to determine the sensitivity of lateral flow testing for asymptomatic SARS-CoV-2 detection in low prevalence settings: Testing frequency and public health messaging is key, PLOS Biology (2021). DOI: 10.1371/journal.pbio.3001216
Journal information: PLoS Biology

Citation: Lateral flow testing should not be used as a green light for activities (2021, April 29) retrieved 18 June 2021 from https://medicalxpress.com/news/2021-04-lateral-green.html
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.
9 shares

Feedback to editors

User comments