Novel, non-invasive method to predict and reduce the relapse of childhood cancers

Novel, non-invasive method to predict and reduce the relapse of childhood cancers
Neuroblastoma CTCs expressing characteristic markers are isolated using cell size-based separation. (A) Schema of experimental design and 3 serial timepoints where blood and/or bone marrow samples were obtained from patients for CTC enrichment. (B) Representative photomicrographs of cytospots of CTC-enriched fractions demonstrating PHOX2B- and TH-positivity of isolated large, atypical cells on immunohistochemical staining (arrows; NBL20 at initial diagnosis and NBL10 following induction chemotherapy, respectively; scale bar: 50 µm), and corresponding bone marrow aspirate with neuroblastoma tumor cell infiltration for size comparison (NBL20 at initial diagnosis; H&E, scale bar: 50 µm). (C) Relative gene expression of PHOX2B and TH in CTC-enriched and waste fractions from blood samples taken at diagnosis. Credit: Frontiers in Oncology (2022). DOI: 10.3389/fonc.2022.939460

A team of scientists and doctors from the KK Women's and Children's Hospital (KKH), Yong Loo Lin School of Medicine, National University of Singapore (NUS Medicine) and the Institute for Health Innovation and Technology has discovered a novel, noninvasive method to predict and reduce the relapse of childhood cancers.

The most common form of solid tumor affecting children is neuroblastoma. It is known to be the cause of a disproportionate number of childhood cancer deaths. The majority of relapsed patients would have a very low chance of survival despite being given the best care.

"The bone marrow is the site where majority of neuroblastoma relapses occur. This discovery provides a simplified method of assessing cancer spread. The current method of sampling bone marrow can be complex, painful and costly. This is particularly daunting for and their families," explained Dr. Amos Loh, Senior Consultant at the Department of Pediatric Surgery, Deputy Chair of the Division of Surgery, and Chair of the Pediatric Brain and Solid Tumor Program, KK Women's and Children's Hospital.

"Where current methods may show patients as cancer-free at the end of treatment, our novel approach may detect 'leftover' disease not identified by current means. This could one day save patients' lives through appropriate early interventions to prevent relapse," added Dr. Loh.

Cancer relapse is usually caused by a small number of "leftover" that remain circulating in the blood long after treatment. These cells can eventually settle in various parts of the body and give rise to multiple tumors, years later.

The new, non-invasive method to monitor neuroblastoma is pain-free and less costly, and involves only a small amount of blood from the patient. The is processed to separate cancer cells from other cells. The cancer cells are then analyzed for genes that would help doctors to determine the likelihood for the cancer to spread to the bone marrow, thereby predicting the likelihood of a relapse. The study identified several genes that predicted relapse in a group of neuroblastoma patients. These include OLFML2B, STAT1, ARHGDIB, STAB1 and TLR2—genes known to be associated with a more aggressive disease state in neuroblastoma.

"We hope that this method can replace current invasive methods such as sampling of in the near future, and be potentially expanded to other childhood cancers," said Associate Professor Chen Zhi Xiong from the Department of Physiology and NUS Centre for Cancer Research at NUS Medicine.

"We are also exploring the use of circulating cancer cells to better understand the biology of neuroblastoma and variations in treatment response to provide more personalized care for patients. Besides cancer cells, we are also investigating other circulating biological entities in blood that may provide further options to monitor treatment effectiveness, and help predict cancer spread and relapse in childhood cancers."

The team hopes that the new method of predicting and reducing the of childhood cancers can soon be put to in the near future.

The study was published in Frontiers in Oncology.

More information: Amos H. P. Loh et al, Pro-metastatic and mesenchymal gene expression signatures characterize circulating tumor cells of neuroblastoma patients with bone marrow metastases and relapse, Frontiers in Oncology (2022). DOI: 10.3389/fonc.2022.939460

Citation: Novel, non-invasive method to predict and reduce the relapse of childhood cancers (2022, October 5) retrieved 23 July 2024 from
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