Breast cancer spread uncovered by new molecular microscopy

Breast cancer spread uncovered by new molecular microscopy
Converting BaSISS spatial signals into maps of clones. a, Bar plots of cancer cell fractions (CCFs) derived from bulk WGS of the P1 samples. b, Phylogenetic tree reconstructed from multiregional bulk WGS data from P1. Each branch is labeled with the total number of WGS mutations defining the branch (gray text) and the number of BaSISS probes designed to target that branch (black text). c, Three heatmaps of variant allele fractions (VAFs) calculated using data derived from n = 11 regions of P1-ER1 and P1-ER2 (marked in d). Raw BaSISS VAFs (for each target mutation the number of mutant signals divided by total number of mutant plus wild-type signals) (top) and model-imputed BaSISS VAFs (middle) are derived from raw BaSISS signal data within these regions. In serial tissue cryosections, corresponding z-stack regions were identified and subjected to LCM–WGS. Resulting LCM–WGS VAFs are presented (bottom). Mean per-gene correlations are approximately 0.41 and 0.90 for BaSISS to LCM–WGS and model-imputed VAFs to LCM–WGS comparisons, respectively. Sample names are colored according to the dominant BaSISS subclone in the sampled region. Each row represents a targeted mutation. The mutations plotted in d are labeled by their gene name; for PTEN there are two separate mutations. d, Spatial BaSISS detections of barcodes reporting on five selected mutations, colored according to their targeted branch. White contours indicate LCM regions (relates to c). e, BaSISS clone maps in physical space projected on the DAPI image (nuclei are white), derived using BaSISS mathematical modeling of signals from 45 informative targets. Each clone has a different color, and dominant clones are reported (shown if the CCF is more than 25% and the inferred local cell density is more than 300 cells per mm2). Scale bars, 2.5 mm (d,e). Credit: Nature (2022). DOI: 10.1038/s41586-022-05425-2

New technology can trace which populations of breast cancer cells are responsible for the spread of the disease, and for the first time highlights how the location of cancer cells could be as important as mutations in tumor growth.

The method was created by a team from the Wellcome Sanger Institute, EMBL's European Bioinformatics Institute (EMBL-EBI), the German Cancer Research Center (Deutsches Krebsforschungszentrum, DKFZ), the Science for Life Laboratory in Sweden, and collaborators. It could be used to help answer some of the big questions in cancer, such as why some cancer cells spread, how treatment resistance is formed, and why some therapies fail.

The new study, published in Nature, shows how it is now possible to map how tumors have developed, combining the cancer cells' genetic information, the surrounding cell types, and how they interact with their environment over time.

In the future, this approach could be used to see how treatments influence the cancer at not only the genetic level, but also any impact on how the tumor interacts with the and the environment around it.

Breast cancer is one of the most common cancers in the UK, with around 55,500 women and around 370 men being diagnosed every year.

Breast cancer commonly starts when cells start to grow uncontrollably, often due to mutations in the cells. Overtime the tumor becomes a patchwork of cells, called cancer clones, each with different mutations. As they are genetically different, they can have different reactions to treatments. For example, some of these cancer cells could become resistant to treatment, or some could spread around the body.

The mutations that occur will be influenced by what is happening around the cancer, the cells that it is surrounded with, and the individual's immune system. Therefore, being able to study the environment that a cancer cell is in, what mutations arise, and which cancer cells spread around the body gives a complete view of tumor evolution.

This new technology, created by researchers from the Wellcome Sanger Institute, EMBL-EBI, DKFZ, the Science for Life Laboratory, and collaborators, uses hundreds of thousands of tiny fluorescent molecular probes to interrogate cellular DNA and RNA and scan large pieces of tissue using fluorescence microscopy. It can genetically and physically map out a cancer's unique set of clones, how their gene expression programs change and show how they interact with their environment.

Artem Lomakin, first author from EMBL's European Bioinformatics Institute (EMBL-EBI) and the German Cancer Research Center (Deutsches Krebsforschungszentrum, DKFZ), said, "We have created a system that combines computational and experimental techniques that allows us to map evolutionary cancer lineages in their natural habitat of human tissue."

"While it has been previously possible to trace the lineage of cancer tumor cells in an experimental setup, this is the first time that multiple lineages were traced in human tissues, giving a complete overview of development in the body. Insights generated by our system were impossible to get before, especially at this scale."

The team found that across multiple stages of breast cancer development there were specific, and often unexpected, patterns of clone growth and that genetic clones behave differently depending on where in the breast they started.

The results also suggest that sometimes it is not only the genetics that influence how cancers grow, but also the location of the tumors. In this case, the genetics can be used as a tool to find out more about what happened in the situation that drove the cancer clones to spread around the body.

Professor Mats Nilsson, co-senior author from the Science for Life Laboratory at Stockholm University, said, "Cancer is caused by genetic mutations in a cell and this research was the first time that we were able to use DNA base specific probes to target dozens of these mutations in a set of cancer cell clones."

"This innovative technique allowed us to accurately reconstruct the spread of these clones. An important insight from our research is that it may not be the genetic changes alone that are the reason that the cancer cells survive and spread; it could also be where they are. This adds an additional layer of complexity as well as new potential ways to target the disease. It may also offer explanations about why some treatments only work in some individuals, even if they have similar mutations to others, as the tumors are found in different areas of the breast."

In the future, it could be possible to develop therapies that could prevent or lessen the cancer's ability to grow and spread, by influencing the environment around the tumor. In addition to this, researchers could use the newly developed tool to test how new treatments affect both the cancer and its interaction with the immune system, giving a complete picture of how therapies work and any possible side effects.

Professor Moritz Gerstung, co-senior author from the German Cancer Research Center (Deutsches Krebsforschungszentrum, DKFZ), said, "Sequencing cancer tumors can give us a good understanding about the genetic diversity in a tumor. But the exciting thing about this technology is that for the first time, we can see how the environment shapes cancer evolution."

"We were able to see which cancer clones progress to become more aggressive, and which don't, and this will enable us to get a much better understanding of what the key steps are in , and how we can lessen or prevent disease."

Dr. Lucy Yates, co-senior author from the Wellcome Sanger Institute, said, "Our research has created a tool that can trace which breast cancer cells go on to cause tumors in other parts of the body, and can help answer some of the big questions in cancer, such as why some spread and some don't."

"To fully understand and therefore treat breast cancer, we need to be able to see the entire picture of how the cancer interacts in the body, with the cells around it, and with the immune system. This new technology combines multiple techniques and expertise to do this, bringing together different approaches to give a complete view of cancer that has not been previously possible."

More information: Lucy Yates, Spatial genomics maps the structure, nature and evolution of cancer clones, Nature (2022). DOI: 10.1038/s41586-022-05425-2

Journal information: Nature

Citation: Breast cancer spread uncovered by new molecular microscopy (2022, November 9) retrieved 8 February 2023 from
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