Gut microbes influence binge-eating of sweet treats in mice

Gut microbes influence binge-eating of sweet treats in mice
Gut microbiota reduce the incentive salience of a high-sucrose reward and decrease activity in mesolimbic brain regions linked to reward behaviors (A) Schematic illustrating timeline of fixed-ratio 1 (FR1) training and progressive ratio (PR) breakpoint testing. (B) High-sucrose pellets obtained during daily FR1 training sessions of VEH (n = 12) and ABX (n = 12) mice. Shown is the mean (± SEM). Significance calculated via two-way repeated measures ANOVA followed by Šidák’s multiple comparisons test. (C) Breakpoints of VEH (n = 10) and ABX (n = 12) mice from the progressive ratio requirement assay. Shown is the mean (± SEM). Significance calculated via two-tailed Student’s t test. (D) Representative images of the nucleus accumbens (NAc) and ventral tegmental area (VTA) in VEH and ABX mice given 1 h of free access to high-sucrose pellets, with c-Fos intensity represented in green. Scale bars are 100 μm. Images are cropped to emphasize the region of interest. (E) Density of c-Fos+ neurons in the NAc shell, NAc core, and VTA (n = 8/group) after 1 h of access to high-sucrose pellets. Shown is the mean (± SEM). Significance calculated via two-way ANOVA with microbiota status and access to high-sucrose pellets as factors, followed by Bonferroni’s multiple comparisons test (within brain regions). Credit: Current Biology (2022). DOI: 10.1016/j.cub.2022.10.066

You just meant to have a single Oreo as a snack, but then you find yourself going back for another, and another, and before you know it, you have finished off the entire package even though you were not all that hungry to begin with.

But before you start feeling too guilty for your gluttony, consider this: It might not be entirely your fault. Now, new research in mice shows that may suppress binge eating behavior.

Oreos and other desserts are examples of so-called "palatable foods"—food consumed for hedonistic pleasure, not simply out of hunger or nutritional need. Humans are not alone in enjoying this kind of hedonism: Mice like to eat dessert, too. Even when they have just eaten, they will still consume sugary snacks if available.

The new Caltech study shows that the absence of certain gut bacteria causes mice to binge eat palatable foods: Mice with microbiotas disrupted by oral antibiotics consumed 50% more sugar pellets over two hours than mice with gut bacteria. When their microbiotas were restored through fecal transplants, the mice returned to normal feeding behavior.

Further, not all bacteria in the gut are able to suppress hedonic feeding, but rather specific species appear to alter the behavior. Binging only applies to palatable foods; mice with or without both still eat the same amount of their regular diet. The findings show that the gut microbiota has important influences on behavior and that these effects can be modulated when the microbiota is manipulated.

The study was led by graduate student James Ousey in the laboratory of Sarkis Mazmanian, Luis B. and Nelly Soux Professor of Microbiology. A paper describing the research appears in the journal Current Biology on November 29.

"The has been shown to influence many behaviors and disease states in mouse models, from sociability and stress to Parkinson's disease," Mazmanian says. "The recent appreciation that feeding behaviors, driven by motivation, are subject to the composition of the gut microbiome has implications not just to obesity, diabetes, and other metabolic conditions but perhaps to overuse of alcohol, nicotine, or illicit substances that bring pleasure."

To examine how the gut microbiota influenced feeding behaviors, Ousey gave a group of mice antibiotics for four weeks, wiping out the animals' . He then compared their feeding behavior to normal mice with a healthy gut microbiota. The two groups ate about the same amount of their standard mouse diet, called chow.

But the real difference was in how much palatable, or dessert-like, food the mice consumed. When presented with high-sucrose pellets, the antibiotic-treated mice ate 50% more pellets over two hours and ate in longer bursts than their healthy mouse counterparts.

Ousey then aimed to determine how much effort the mice were willing to expend to obtain sugary snacks. In another set of experiments, instead of simply having treats placed in their cages, the mice needed to push a button to receive a pellet. Each subsequent pellet required the mice to push the button more and more times. The untreated mice, at some point, would lose interest in pushing the button and wander away. However, the mice given oral antibiotics expended much more effort to obtain more and more sugar, pressing the button repeatedly as if desperately craving a snack.

Importantly, this binge eating behavior is actually reversible: The researchers could return the mice back to normal feeding behavior simply by restoring the mouse microbiota through a fecal transplant. The restored mice still consumed sugar when available but did not exhibit the same overeating behavior.

The gut microbiota contains hundreds of bacterial species, and the team suspected that some were more influential than others in driving the binge eating behavior.

"To tease out which specific microbes might be involved, I gave different cohorts of mice different antibiotics individually," Ousey explains. "The different antibiotics target different bacteria. What I observed was that mice given either ampicillin or vancomycin, but not neomycin or metronidazole, overconsume these high-sucrose pellets compared to controls. That would suggest that there's some microbe, or some collection of microbes, that is susceptible to either ampicillin or vancomycin, which is responsible for controlling the normal response to the highly palatable foods."

The team then identified that increased levels of bacteria from the family S24-7 (a type of bacteria specific to lab mice) and from the genus Lactobacillus were associated with reduced overconsumption. When these were given to the antibiotic-treated mice, but not other bacteria, hedonic feeding was suppressed.

Though the study only draws conclusions about the mouse microbiota, it opens up new directions of study for understanding how and why we may be driven to overconsume sugary snacks. "I think it would be so intriguing to see if people given exhibit differences in their eating patterns and dietary choices, and whether these things can be associated with the gut microbiota," says Ousey.

"We know that humans with eating disorders like binge eating disorder and anorexia nervosa have differences in their gut microbiota compared to humans that are not diagnosed with these conditions. Obviously, perhaps the eating disorder affects the because they're eating different foods; perhaps it's bidirectional. But investigations into how antibiotics might affect the responses to palatable foods in humans are definitely doable."

"We do not understand the neurobiology underlying the observation that the microbiome impacts overconsumption of palatable foods in ," says Mazmanian. "Future studies in our lab and others will explore the gut–brain axis in modulating reward circuits in the brain as well as possibly devising probiotics to intervene in eating disorders."

More information: James Ousey et al, Gut microbiota suppress feeding induced by palatable foods, Current Biology (2022). DOI: 10.1016/j.cub.2022.10.066

Journal information: Current Biology

Citation: Gut microbes influence binge-eating of sweet treats in mice (2022, November 30) retrieved 4 February 2023 from https://medicalxpress.com/news/2022-11-gut-microbes-binge-eating-sweet-mice.html
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