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A gutsy move: New study challenges conventional wisdom about nerve cell origins of 'the second brain'

A gutsy move – new study challenges conventional wisdom about nerve cell origins of “the second brain”
Half of all adult small intestinal myenteric neurons are derived from a non-neural crest lineage. Credit: (2023). DOI: 10.7554/eLife.88051.1

"Following your gut;" "losing your appetite;" "a gutsy move." We may think of the gut as a simple digestive organ, but these familiar phrases reflect the central role the gut plays in a much wider range of essential functions. The entire digestive tract is lined by the enteric nervous system (ENS), a vast network of millions of neurons and glial cells—the two primary cell types also found in the central nervous system. While often called the second brain, the ENS produces the same neurotransmitters and originated much before the central nervous system in the brain evolved.

The functions of the ENS are crucial to life and extend far beyond digestion, as it regulates immunity and gut secretions, and enables complex, bi-directional communication between the gut and the brain. This is why a happy gut co-exists with a happy , and why digestive issues can lead to changes in mood and behavior.

Since the mid-20th century, scientists have believed that the ENS is derived from the before birth and remains unchanged after. Now, in a paper published in the journal eLife, researchers at Beth Israel Deaconess Medical Center (BIDMC) present a completely new paradigm describing a developmental pathway by which ENS development continues after birth in mice and human tissue samples. This discovery overturns decades of scientific dogma on the fundamental biology of neuroscience and of ENS, by showing evidence for the first time of a non-ectodermal and a mesodermal origin for large numbers of enteric neurons born after birth. The findings show the relevance of these neurons to the maturation and aging of the ENS in health and disease.

"These results indicate for the first time that the mesoderm is an important source of neurons in the second largest nervous system of the body," said Subhash Kulkarni, Ph.D., a staff scientist at BIDMC and an assistant professor in the Division of Medical Sciences at Harvard Medical School. "How we mature and how we age is central to our understanding of health and disease in our rapidly aging population. The increasing proportion of neurons of mesodermal lineage is a natural consequence of maturation and aging; further, this lineage can be expected to have distinct vulnerabilities to disease."

Using transgenic mice models, and genetic analyses, Kulkarni and colleagues analyzed the ENS neuronal populations in adult mice and human tissues. Using mice models, the team found that while the early post-natal ENS cells were from the expected neural crest lineage, that pattern changed rapidly as the animal matured. Kulkarni and colleagues documented the arrival and continual expansion of a novel population of enteric neurons that were derived from the mesoderm—the same lineage that gives rise to the muscle and heart cells. This newly discovered population of mesoderm-derived neurons expanded with age, such that they comprised a third of all enteric neurons in adolescent mice, half of all enteric neurons in adult mice, and then eventually outnumbered the original neural crest-derived population of enteric neurons in aging mice.

By assessing the molecular signature of these neurons, the team identified new cellular markers which were used to identify this population of mesoderm-derived neurons in human gut tissue. These markers also provided pharmacological targets, which the researchers used to not only manipulate the proportions of the mesodermal neurons in adolescent mice, but also to reduce their dominant proportions in the aging mouse gut to cure age-associated slowing of gut movement.

"We can now work to understand how these findings can be translated into human systems to provide a disease modifying cure to aging patients whose chief complaint often includes diseases of the GI tract," added Kulkarni. "By reversing one of the biggest dogmas of neuroscience, we are now in uncharted territory, and at the same time, have a huge opportunity to understand this hidden basic, translational, and clinical biology of neurons. The newly discovered lineage of neurons presents us with potential new drug targets that could help large populations of patients."

Co-authors included Monalee Saha, Jared Slosberg, Alpana Singh, Sushma Nagaraj, Chengxiu Zhang, Alicia Bukowski, Zhuolun Wang, Guosheng Liu, Jenna Leser, Mithra Kumar, Shriya Bakhshi, Elizabeth Vincent, and Loyal A. Goff of Johns Hopkins University School of Medicine; Laren Becker and of Stanford University School of Medicine; Matthew Anderson and Mark Lewandoski of Center for Cancer Research, National Cancer Institute; and Pankaj Jay Pasricha of the Mayo Clinic.

More information: Subhash Kulkarni et al, Age-associated changes in lineage composition of the enteric nervous system regulate gut health and disease, eLife (2023). DOI: 10.7554/eLife.88051.1

Journal information: eLife

Citation: A gutsy move: New study challenges conventional wisdom about nerve cell origins of 'the second brain' (2023, August 9) retrieved 3 December 2023 from
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