This article has been reviewed according to Science X's editorial process and policies. Editors have highlighted the following attributes while ensuring the content's credibility:


peer-reviewed publication

trusted source


Tissue samples show the deep genetic and cellular impacts of smoking

Credit: CC0 Public Domain

It's no secret that smoking is extremely detrimental to health. Tobacco smoke contains thousands of chemicals, including carcinogens, increasing the risk of cancer, cardiovascular and respiratory diseases.

A new study from the University of Chicago analyzed data from more than 900 samples of nine different human tissue types to understand just how deep the cellular and genetic damage from smoking goes. The research team generated epigenetic data to assess the effects of smoking on DNA methylation, or genetic locations where a handful of atoms can attach to DNA and turn off .

They found several new regions associated with smoking, including some that are shared across tissue types, suggesting that DNA methylation is part of the body's attempts to defend itself from the damaging effects of .

The paper, "The association of cigarette smoking with DNA methylation and gene expression in human tissue samples," was published March 14, 2024, in the American Journal of Human Genetics.

Exposures affect more than the blood

Epidemiological studies on the effects of exposure to chemicals and other environmental contaminants like smoke often use only , mostly because they are easy to collect. For this new study, senior author Brandon Pierce, Ph.D., Professor of Public Health Sciences and Human Genetics at UChicago, and his team worked with samples from the GTEx Project, a biobank housing human tissue samples from more than 950 postmortem tissue donors. The team used DNA methylation data from nine tissue types, including lung, colon, ovary, prostate, whole blood, breast, testis, kidney, and muscle.

"If we want to understand the effects of environmental exposures like smoking, it's important to study a variety of tissue types, in addition to blood, because disease occurs in many different organs in the body," Pierce said. "The epigenome varies dramatically across and tissue types, as could the epigenetic effects of exposures."

The also came with data about whether the donor ever smoked in their lifetime (i.e. "ever smokers"), if they never smoked (never smokers), or they were currently smoking at the time of death (current smokers).

The team analyzed data on DNA methylation at cytosine-guanine (CpG) dinucleotides, or regions of DNA where a cytosine nucleotide is followed by a guanine nucleotide in the sequence of bases. They found 6,350 smoking associated CpGs in , and 2,753 in colon tissues, meaning that these genetic regions showed differences in DNA methylation among the ever or current smokers, compared to never smokers.

Doing this same kind of analysis in blood samples only reflects the effects of smoking on , whereas analyses in non-blood tissues gives researchers a new window into its health impacts.

"When we do our multi-tissue study, it gives a way more comprehensive understanding of how smoking affects the human body," said James Li, the study's co-first author and an MD/Ph.D. student in the Department of Public Health Sciences. "I think our work is super exciting because we had the power to directly examine the effects of smoking in tissues such as lung and colon, which are highly relevant in the pathophysiology of smoking."

Understanding defense mechanisms to smoke

Many of the CpGs identified in the tissues matched up with ones identified in previous studies using blood samples, including genes involved in detoxification or transformation of foreign chemicals so they can be removed from the body. There were big differences among too, however.

The lungs have nearly three times as many smoking-associated CpGs identified as the colon, for example, which makes sense because the lungs are directly exposed to .

"Each tissue is exposed to smoking in a different way. The lung is much more direct through inhalation, whereas the colon comes later. So, that's a different nature of exposure, and we can capture the effects of that difference," said Niyati Jain, co-first author and a Ph.D. student in the Committee on Genetics, Genomics, and Systems Biology.

While we certainly don't need more evidence that smoking is bad for health, this -specific understanding of its effects does help understand what's happening at a cellular level, and how the body responds to exposure.

"The epigenetic responses to smoking may reflect mechanisms that defend us against or mediate the adverse effects of smoking. Characterizing them can give us a better understanding of disease relevance and risk," Jain said.

In addition to smoking, Pierce said he can envision using the same technique to understand other, less obvious, environmental exposures. "We can identify epigenetic signatures that can serve as biomarkers of exposure effects. For example, based on a person's epigenome, we can potentially tell if they've been exposed to a particular type of contaminant," he said. "The more we can increase the diversity of tissues and number of samples from more people, the more we can learn."

More information: The association of cigarette smoking with DNA methylation and gene expression in human tissue samples, The American Journal of Human Genetics (2024). DOI: 10.1016/j.ajhg.2024.02.012.

Citation: Tissue samples show the deep genetic and cellular impacts of smoking (2024, March 14) retrieved 29 May 2024 from
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.

Explore further

Large-scale study explores link between smoking and DNA changes across six racial and ethnic groups


Feedback to editors