Antibody engineering know-how

June 5, 2013, University College Dublin
Antibody engineering know-how
Credit: IgG and engineered recombinant antibody fragments

(Medical Xpress)—Antibodies are of enormous value to society as therapeutic and diagnostic agents. There are many scientists worldwide interested in producing their own novel engineered antibody based molecules as part of their research work.

Scientists from UCD Conway Institute and UCD Centre have published an accessible instruction manual detailing how to make antigen binding molecules for molecular studies and translational research.

This protocol published in the journal Nature Protocols describes the design and development of recombinant antigen-binding molecules from through rapid identification and cloning of variable genes.

Describing the work, Conway scientist Dr David O'Connell said, "The protocol is accessible for groups not specialised in this area, and allows them to carry out reverse engineering of functional, recombinant antigen-binding molecules from hybridomas within 50 working days.

We are delighted with this publication and view it an endorsement by a prestigious scientific journal of our experience in the engineering of . It reflects our capabilities in antibody design, construction and characterisation that are made possible through the excellent infrastructure available on the university campus."

This research reflects a successful collaboration between UCD scientists with CRO Genscript Inc. The team, which also includes Conway Fellow, Professor Gil Lee hopes to attract new collaborators with both industry and academia on foot of this work.

Explore further: New stable antibodies created

More information: Nat Protoc. 2013 May 16;8(6):1125-48. doi: 10.1038/nprot.2013.057. Epub 2013 May 16.

Related Stories

New stable antibodies created

June 29, 2012
(Medical Xpress) -- Australian scientists have overcome one of the most pressing problems facing the pharmaceutical industry – how to create antibodies that are stable enough to meet stringent requirements necessary ...

Reducing experimental inflammatory arthritis

May 24, 2013
(Medical Xpress)—UCD researchers led by Conway Fellow, Professor David Brayden in UCD School of Veterinary Medicine have successfully reduced inflammation in the swollen arthritic knees of a murine model using a novel nanoparticle.

Evolution in the antibody factory: How immune cells are able to advance their own evolution

March 11, 2013
Immune system B cells play a crucial role in the defence of pathogens; when they detect such an intruder, they produce antibodies that help to combat the enemy. They concurrently and continuously improve these molecules to ...

Peptides for the treatment of severe diseases

April 15, 2013
A new class of drugs for the treatment of severe diseases such as cancer and autoimmune diseases is developed by the start-up Bicycle Therapeutics. The company is generating bicyclic peptides that can selectively bind disease-related ...

Recommended for you

Secrets of longevity protein revealed in new study

January 17, 2018
Named after the Greek goddess who spun the thread of life, Klotho proteins play an important role in the regulation of longevity and metabolism. In a recent Yale-led study, researchers revealed the three-dimensional structure ...

Weight flux alters molecular profile, study finds

January 17, 2018
The human body undergoes dramatic changes during even short periods of weight gain and loss, according to a study led by researchers at the Stanford University School of Medicine.

The HLF gene protects blood stem cells by maintaining them in a resting state

January 17, 2018
The HLF gene is necessary for maintaining blood stem cells in a resting state, which is crucial for ensuring normal blood production. This has been shown by a new research study from Lund University in Sweden published in ...

Magnetically applied MicroRNAs could one day help relieve constipation

January 17, 2018
Constipation is an underestimated and debilitating medical issue related to the opioid epidemic. As a growing concern, researchers look to new tools to help patients with this side effect of opioid use and aging.

Researchers devise decoy molecule to block pain where it starts

January 16, 2018
For anyone who has accidentally injured themselves, Dr. Zachary Campbell not only sympathizes, he's developing new ways to blunt pain.

Scientists unleash power of genetic data to identify disease risk

January 16, 2018
Massive banks of genetic information are being harnessed to shed new light on modifiable health risks that underlie common diseases.

0 comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.