Promising new target may treat pulmonary fibrosis

Promising new target may treat pulmonary fibrosis
Transgenic mouse model was used to target lung mesenchymal cells (green). Credit: The Saban Research Institute of Children's Hospital Los Angeles

By uncovering the mechanism by which fibrous tissue cells in the lung multiply, researchers at The Saban Research Institute of Children's Hospital Los Angeles (CHLA), along with colleagues in Mexico and Canada, have identified a promising new approach for the treatment of pulmonary fibrosis.

Idiopathic or IPF- where the disease appears spontaneously and without a known cause - is a severe, in which the rapid growth of results in increasingly severe breathing difficulties and a high mortality rate.

Excessive signaling of the growth factor TGF-β is recognized as a central player in , and its increased expression has been identified in both IPF patients and experimental models of the disease. However, in addition to its pro-fibrotic effect, TGF-β signaling is essential to aspects of other systems, such as appropriate immune response.

Therefore, complete therapeutic blockade of TGF-β pathway isn't ideal, says the study's principal investigator Wei Shi, MD, PhD, of the Developmental Biology and Regenerative Medicine research program at CHLA. "This is a very complicated signaling pathway, which promotes mesenchymal stem cell proliferation, while also acting as a tumor suppressant," said Shi, who is also an associate professor in the Department of Surgery, Keck School of Medicine of the University of Southern California. "Our goal was to find a way to target specific cells that result in without affecting other cells."

Using a unique transgenic mouse model developed at The Saban Research Institute, the researchers were able to manipulate TGF-β signaling in adult lung mesenchymal cells at selected stages of lung fibrosis. They showed that the progression of fibrosis mediated by TGF-β was independent of early inflammatory processes during initiation of fibrosis - an understanding that is vital in designing therapeutic strategies to stop its progression in IPF patients.

Secondly, they identified a downstream gene called P4HA3 that is important to the overabundant deposition associated with IPF. This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis. Inhibition of this enzyme was shown to reduce the TGF-β-stimulated collagen production in both cultured fibroblasts (connective tissue cells that produces collagen) and mouse models of lung fibrosis.

"Our data indicate that increased expression of collagen prolyl hydroxylase is one of the important mechanisms underlying the proliferation of fibrous tissue that is mediated by TGF-β. Inhibiting this enzyme appears to be a promising therapy to interfere with excessive collagen production and deposition in IPF patients," Shi concluded.

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Citation: Promising new target may treat pulmonary fibrosis (2015, March 18) retrieved 25 October 2020 from
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