New findings give insight into the cell membrane repair process of torn muscle fibers

New findings give insight into the cell membrane repair process of torn muscle fibers
Scavenger cell removes the repair patch. Scale bar: 4 µm. Credit: Volker Middel / KIT

Everybody knows the burning sensation in the legs when climbing down a steep slope for a long time. It is caused by microruptures in the cell membrane of our muscle fibers. These holes in the cell envelopes must be closed as soon as possible as otherwise muscle cells will die off. Researchers at KIT were able to observe this repair process using high-resolution real-time microscopy. It only takes a few seconds until proteins from the inside of the injured cell form a repair patch that finally closes the hole in the membrane. The researchers at KIT now demonstrated that scavenger cells moving around within the muscle virtually perform nano-surgery to remove this repair patch later and restore the normal cell membrane structure.

The cells of our skeletal muscles have effective mechanisms for the repair of ruptures in their cell membranes. These ruptures are due to mechanical stress to which we expose our muscles even when doing healthy exercises. The is an important barrier which is essential to the proper functioning and survival of cells. If this barrier collapses and cannot be repaired quickly, the muscle cell will die, resulting in a loss of . People whose , e.g. dysferlin, do not work properly develop atrophy of the muscles which leads to most severe disabilities and premature death.

In an interdisciplinary cooperation project of the KIT research teams led by Uwe Strähle and Gerd Ulrich Nienhaus, the PhD students Volker Middel and Lu Zhuo developed new techniques to observe membrane repair processes with ultra-high resolution in real time in human cells and in of zebrafish embryos. They proved that the repair patch assembling itself from repair proteins, such as dysferlin or annexines, also accumulated the phosphatidylserine lipid. Phosphatidylserine is a known appetizer for , the so-called macrophages.

The KIT researchers presented a movie that shows how the macrophages indeed latch to the repair patch and eat it up. Only after the patch has been removed, the cell envelope is fully restored. Thus, the repair of the membrane in requires, in addition to the formation of repair patches in the injured cell, the aid of macrophages roaming around within the muscle. The researchers further demonstrated that a short amino acid sequence in the dysferlin repair protein is responsible for the phosphatidylserine transport. It is remarkable that there are myopathy patients who have a defect precisely in this sequence of the dysferlin protein. The new findings may therefore contribute to the development of therapies against muscle atrophy.


Explore further

Missing protein restored in patients with muscular dystrophy

More information: Volker Middel et al. Dysferlin-mediated phosphatidylserine sorting engages macrophages in sarcolemma repair, Nature Communications (2016). DOI: 10.1038/NCOMMS12875
Journal information: Nature Communications

Citation: New findings give insight into the cell membrane repair process of torn muscle fibers (2016, September 21) retrieved 21 October 2019 from https://medicalxpress.com/news/2016-09-insight-cell-membrane-torn-muscle.html
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.
5 shares

Feedback to editors

User comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more