Researchers from Kanazawa University report in the Journal of Neuroscience on a microdialysis study on mice to determine mechanisms underlying the inflammatory response in the brain associated with fever. Their findings might be used to develop new strategies for treatment.
The onset of fever is associated with the release in the hypothalamus of a lipid compound called prostaglandin E2 (PGE2), which has an important role in the regulation of body temperature. However, the mechanism of PGE2 release, and the role of membrane transporters (in particular of the prostaglandin transporter OATP2A1, encoded by the gene SLCO2A1) in this process was unknown.
To shed light on this question, Takeo Nakanishi at Kanazawa University, Japan, and colleagues performed a microdialysis study on mice. The researchers used mice with normal Slco2a1, mice with total Slco2a1 deficiency, and mice with monocyte-/macrophage-specific Slco2a1 deficiency. They first injected them with physiological saline, observing the same body temperature for mice with and without SLCO2A1, indicating that the presence of OATP2A1 does not affect the basal body temperature. They then administered lipopolysaccharide, a pyrogen that normally causes fever. Mice with Slco2a1 developed a fever after two hours, whereas the pyrogenic effect of lipopolysaccharide was not observed in mice with total SLCO2A1 deficiency. They further demonstrated that the body temperature of mice with monocyte-/macrophage-specific Slco2a1 deficiency was partially attenuated. Intriguingly, an inhibitor of OATP2A1 injected to the brain of rats with normal Slco2a1 inhibited the febrile response—in this case, only an initial rise in body temperature was observed.
The study reveals that the onset of fever is associated with increased PGE2 concentration in the hypothalamus interstitial fluid, but not in the cerebrospinal fluid, thus OATP2A1 seems to work by maintaining high levels of PGE2 in the hypothalamus, either by stimulating its secretion from glial cells in the hypothalamus and from brain capillary endothelial cells, or by facilitating its transport through the blood-brain barrier. OATP2A1 seems to be involved in the secretion of PGE2 from macrophages, but OATP2A1 in cells other than macrophages may also contribute to the febrile response.
This new insight into the mechanisms underlying the inflammatory response in the brain associated with fever might be used to develop new strategies for treatment, pointing to OATP2A1 as a useful therapeutic target.
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Yoshinobu Nakamura et al. Prostaglandin transporter OATP2A1/SLCO2A1is essential for body temperature regulation during fever, The Journal of Neuroscience (2018). DOI: 10.1523/JNEUROSCI.3276-17.2018