Blood vessels communicate with sensory neurons to decide their fate

Blood vessels communicate with sensory neurons to decide their fate
Blood vessel phyllopods (magenta) touching sensory neurons (green), which also emit phylopods into the blood vessels. These images can be seen thanks to the use of transgenic lines that mark each cell type with fluorescent proteins of different colors. Credit: Universitat Pompeu Fabra - Barcelona

Researchers at Pompeu Fabra University have shown for the first time that blood vessels communicate with neurons in the peripheral nervous system, regulating their proliferation and differentiation. The study is published today in the journal Cell Reports and was conducted using zebrafish as a model. It was led by Berta Alsina, principal investigator of the Morphogenesis and Cell Signaling in Sensory Systems group, and involved Laura Taberner and Aitor Bañón.

The researchers, using real-time videos, have discovered that both the and the cells of emit dynamic protrusions to be able to 'talk' to each other. These protrusions are called signaling filopodia or cytonemes and they have a receptor or ligand at the tip that allows them to send signals. It was only very recently discovered and it is a highly precise signaling mechanism, both in space and in time.

"It was known that cells and in the brain communicate but this is the first time it has been witnessed through cytonemes in the peripheral nervous system," Berta Alsina explains. "By using high resolution spatiotemporal visualization techniques in vivo we have seen them in real time and they might also be in the brain," she adds.

This communication allows maintaining some precursors of neurons in quiescence, i.e., dormant, and they constitute a reservoir of stem cells. So, if later on in adulthood an injury occurs, quiescent cells can be activated and replace damaged neurons.

In this time-lapse video is shown the development of a zebrafish embryo for 12 hours in which the blood vessels are marked in magenta and the sensory neurons in green, which allows us to observe the interaction of these two cell types. Credit: Laura Taberner, Pompeu Fabra University.

Laura Taberner, first author of the study, explains that "if all neuronal precursors proliferated and differentiated we would not have this reservoir and there would not be the opportunity for regeneration. In the auditory and vestibular system, which is what we are studying, cases of deafness or vertigo may arise."

The study also concludes that the precursors are initially in a hypoxic environment, i.e., lacking oxygen, which keeps them proliferating. When vessels connect to each other during development, oxygen is transported by the blood vessels and the environment becomes normoxic. The researchers have found that oxygen is the second signal of the vessels and in this case, instead of regulating quiescence, oxygen regulates the differentiation of neuronal precursors to neurons.

This study shows that during the development of the , the formation of new neurons and the maintenance of stem cells is highly dependent on signals from the blood vessels. Neurons receive signals from all the surrounding , which are part of the environment in which they reside and the vessels are part of this niche. "This new knowledge will help understand the connection between hearing loss and cardiovascular diseases, as well as improve protocols for in vitro differentiation of neurons for regenerative therapies," Taberner adds.

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More information: Laura Taberner et al, Sensory Neuroblast Quiescence Depends on Vascular Cytoneme Contacts and Sensory Neuronal Differentiation Requires Initiation of Blood Flow, Cell Reports (2020). DOI: 10.1016/j.celrep.2020.107903
Journal information: Cell Reports

Provided by Universitat Pompeu Fabra - Barcelona
Citation: Blood vessels communicate with sensory neurons to decide their fate (2020, July 16) retrieved 30 July 2021 from
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