This article has been reviewed according to Science X's editorial process and policies. Editors have highlighted the following attributes while ensuring the content's credibility:


peer-reviewed publication

trusted source


Neuronal activity shapes the development of astrocytes, shows study

Credit: Pixabay/CC0 Public Domain

Researchers at Baylor College of Medicine have unraveled the processes that give astrocytes, the most abundant glial cell in the brain, their special bushy shape, which is fundamental for brain function. They report in the journal Nature that neuronal activity is necessary and sufficient for astrocytes to develop their complex shape, and interrupting this developmental process results in disrupted brain function.

"Astrocytes play diverse roles that are vital for proper ," said first author Yi-Ting Cheng, a graduate student in Dr. Benjamin Deneen's lab at Baylor. "For instance, they support the activity of other essential brain cells, neurons; participate in the formation and function of synapses, or neuron-to-neuron connections; release neurotransmitters, chemicals that mediate neuronal communication; and make the ."

In the adult brain, the bushy shape of is fundamentally linked to effective brain function. The ends of the branched-out astrocyte structure interact with neurons and regulate synaptic activity.

"If astrocytes lose their structure, then synapses do not behave properly and brain function goes awry," said Deneen, professor and Dr. Russell J. and Marian K. Blattner Chair in the Department of Neurosurgery and director of the Center for Cancer Neuroscience at Baylor. He also is the corresponding author of the work.

"Figuring out how astrocytes acquire their complex, bushy structure is essential to understanding how the brain develops and functions and may bring new insight into how neurodevelopmental conditions emerge. In this study, we investigated the cells and processes that direct the development of astrocyte structure."

Neurons lead the way

When astrocytes develop, neurons are already present and active, so do neurons influence how astrocytes acquire their complex shape?

"We artificially activated or silenced neurons and determined whether this would speed up or slow down astrocyte maturation," Cheng said. "We found that is both necessary and sufficient to drive full astrocyte maturation into a bushy-shaped cell."

So how are astrocytes receiving the signals that direct them down to the proper maturation path? Through several experimental approaches the team discovered that neurons produce a neurotransmitter called GABA that binds to astrocytes via a molecule on their surface named GABAB receptor.

"We knocked out the GABAB receptor in astrocytes and activated the neurons. In this situation, the neurons did not promote the development of a typical astrocyte shape, supporting the idea that neurons communicate with astrocytes via the GABAB receptor to promote their maturation."

"This finding was surprising and very interesting," Deneen said. "Neurotransmitters such as GABA are known to signal between neurons at synapses, but we discovered that neurotransmitters also signal astrocytes, influencing their development by triggering changes in their structure."

Other experiments revealed more pieces of the puzzle of how neurons lead astrocytes to develop their bushy shape. "Neurons produce GABA, which binds to astrocytes via the GABAB receptor. In turn, this activates a series of events, including triggering the expression of another receptor called Ednrb, which drives pathways that remodel cellular architecture inside the cells associated with cell shape," Cheng said.

The researchers also investigated another mystery related to astrocyte development. They found that regulation of the expression of GABAB receptor in astrocytes does not occur in the same way in different brain regions. "This result was totally unexpected," Deneen said. "The GABAB receptor is universally required for astrocytes to develop their bushy shape in all brain regions. How is it regulated differently in different areas of the brain?"

Through bioinformatics analyses the researchers discovered that this regional regulation is conferred by two proteins, LHX2 in the brain cortex and NPAS3 in the through their region-specific interactions with proteins SOX9 and NFIA, which are present in all astrocytes where they regulate GABAB receptor expression. In the cortex, LHX2 only binds to NFIA, while in the olfactory bulb NPS3 only binds to SOX9, enabling each one to regulate GABAB receptor expression in a specific region.

Altogether, the findings suggest that development and function involve a complex pattern of events and proteins triggered by the activity of and that operate in a region-specific manner.

More information: Yi-Ting Cheng et al, Inhibitory input directs astrocyte morphogenesis through glial GABABR, Nature (2023). DOI: 10.1038/s41586-023-06010-x

Journal information: Nature

Citation: Neuronal activity shapes the development of astrocytes, shows study (2023, April 26) retrieved 23 September 2023 from
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.

Explore further

Social isolation triggers astrocyte-mediated deficits in learning and memory


Feedback to editors