Fast track to vascular disease
In Western societies, atherosclerosis of the arteries is one of the leading causes of death. Chronic, localized inflammation of the blood vessel wall facilitates the growth of fibrous plaques, which leads to narrowing or occlusion of the vessel, and thereby promotes heart attacks and stroke. The persistence of the inflammatory reaction is due to a loss of control over the activity of the immune system.
So-called dendritic cells are known to play a central role in the adaptive immune response, functioning as activators of other classes of immune cells. Their precise contribution to the pathogenesis of atherosclerosis has, however, been unclear. An international team of researchers led by Professor Christian Weber of Ludwig-Maximilians-Universität (LMU) Munich and Privatdozentin Alma Zernecke of Würzburg University has now demonstrated that dendritic cells release the chemokine CCL17 as a signal molecule, which inhibits a feedback mechanism that normally limits the activity of the immune system. This explains why the normally transient inflammatory response is not turned off and becomes chronic. The new study has also identified a potential antidote to the signal molecule. As Professor Weber reports, "We were able to prevent the progression of atherosclerosis using an antibody raised against CCL17." In other words, CCL17 offers a promising starting point for the development of new therapeutic strategies.
(Journal of Clinical Investigation, 1 June 2011)
Atherosclerosis can become manifest in various ways. In particular, plaques may suddenly rupture, promoting the formation of a clot that occludes the vessel. If this afflicts the vessels of the heart or brain the result is a heart attack or a stroke. The narrowing of vessels caused by plaque growth can also lead to reduction of the blood supply to the lower limbs and to so-called vascular dementia. Atherosclerosis is initiated by lipid accumulation that occurs directly beneath the inner lining of the blood vessel, and can induce and chronically perpetuate an inflammatory response. Cells of the immune system migrate to the site of the primary lesion and release signaling molecules that attract other types of immune cells which facilitate plaque formation. The atherosclerotic plaques in turn serve as sources of additional signals and mediators, the inflammation reaction gets out of control and continues unchecked, and plaque growth progressively obstructs the flow of blood.
So-called dendritic cells are responsible for determining the targets against which the immune response is directed. They do so by using fragments of target-specific proteins displayed in a molecular complex on their surfaces to activate immune cells called T cells. The T cells have highly variable receptors that enable them to recognize such complexes and to identify the intact target. "Dendritic cells are detectable in arteries and in atherosclerotic tissue, but their precise function in the pathogenesis of plaques has been unclear," explains Weber. "We were particularly interested in learning more about the role of the chemokine CCL17 - a signal protein that is specifically synthesized by mature dendritic cells - in the activation of T cells and in regulating T cell homeostasis."
For their experiments the researchers made use of a transgenic mouse strain in which the gene for CCL17 had been replaced by a DNA sequence encoding the genetic information for the Green Fluorescent Protein (GFP). Thus, in this strain, GFP acts as a proxy for CCL17-producing cells. Since GFP can be visualized by virtue of its fluorescence, the team was able to determine where CCL17-producing cells would normally congregate. This approach also allowed them not only to work out the effects of the lack of CCL17, but also to use advanced methods of microscopy, in particular multiphoton microscopy, to follow how the dendritic cells intrude and accumulate in atherosclerotic plaques and interact with T cells.
In further experiments, the researchers destroyed the T cells of normal mice and replaced them with T cells from the CCL17-deficient mice. Conversely, regulatory T cells were specifically removed from CCL17-deficient mice. "The results of these investigations led us to conclude that CCL17 drives atherosclerosis by inhibiting an important regulatory circuit that acts to restrain the immune response," says Weber. Under normal circumstances, immune reactions are attenuated by regulatory T cells (Tregs) that inhibit other types of immune cells and so ensure that the whole system returns to a balanced state. The new study shows that, in the presence of CCL17, fewer Tregs in the inflamed tissue are activated. In effect, CCL17 disables the Treg-dependent braking mechanism.
"This finding makes CCL17 a promising target for new therapeutic approaches," says Weber, who is anxious to ensure that the results of basic research are transferred as quickly as possible into the clinical setting and find practical medical application. In this case, the first step has already been taken. Using an antibody against CCL17, the authors of the new study were able to block the action of the signal and prevent the progression of atherosclerosis. CCL17 therefore offers a possible handle for new approaches to treatment of the condition. In the next step, Weber and his colleagues hope to clarify the function of the CCL17 receptors on the plasma membrane of the regulatory T cells.
More information: doi:10.1172/JCI4492
Provided by Ludwig-Maximilians-Universität München
- Inflammation worsens danger due to atherosclerosis Jan 22, 2009 | not rated yet | 0
- New atherosclerosis vaccine gives promising results May 06, 2010 | not rated yet | 0
- Nicotinic acid blocks immune cells in atherosclerosis Feb 07, 2011 | not rated yet | 0
- Dendritic cells as a new player in arteries and heart valves Feb 17, 2009 | not rated yet | 0
- New origin found for a critical immune response Mar 01, 2009 | not rated yet | 0
- Motion perception revisited: High Phi effect challenges established motion perception assumptions Apr 23, 2013 | 3 / 5 (2) | 2
- Anything you can do I can do better: Neuromolecular foundations of the superiority illusion (Update) Apr 02, 2013 | 4.5 / 5 (11) | 5
- The visual system as economist: Neural resource allocation in visual adaptation Mar 30, 2013 | 5 / 5 (2) | 9
- Separate lives: Neuronal and organismal lifespans decoupled Mar 27, 2013 | 4.9 / 5 (8) | 0
- Sizing things up: The evolutionary neurobiology of scale invariance Feb 28, 2013 | 4.8 / 5 (10) | 14
How can there be villous adenoma in colon, if there are no villi there
13 hours ago As title suggest. Thanks :smile:
How can there be a term called "intestinal metaplasia" of stomach
May 21, 2013 Hello everyone, Ok Stomach's normal epithelium is simple columnar, now in intestinal type of adenocarcinoma of stomach it undergoes "intestinal...
Pressure-volume curve: Elastic Recoil Pressure don't make sense
May 18, 2013 From pressure-volume curve of the lung and chest wall (attached photo), I don't understand why would the elastic recoil pressure of the lung is...
If you became brain-dead, would you want them to pull the plug?
May 17, 2013 I'd want the rest of me to stay alive. Sure it's a lousy way to live but it beats being all-the-way dead. Maybe if I make it 20 years they'll...
MRI bill question
May 15, 2013 Dear PFers, The hospital gave us a $12k bill for one MRI (head with contrast). The people I talked to at the hospital tell me that they do not...
Ratio of Hydrogen of Oxygen in Dessicated Animal Protein
May 13, 2013 As an experiment, for the past few months I've been consuming at least one portion of Jell-O or unflavored Knox gelatin per day. I'm 64, in very...
- More from Physics Forums - Medical Sciences
More news stories
Every day, their baby stopped breathing, his collapsed bronchus blocking the crucial flow of air to his lungs. April and Bryan Gionfriddo watched helplessly, just praying that somehow the dire predictions ...
Medical research 3 hours ago | 5 / 5 (1) | 0
The human gut is loaded with commensal bacteria – "good" microbes that, among other functions, help the body digest food. The gastrointestinal tract contains literally trillions of such cells, and yet the ...
Medical research 7 hours ago | 5 / 5 (1) | 0 |
Swiss scientists reveal the mechanism responsible for aging hidden deep within mitochondria—and dramatically slow it down in worms by administering antibiotics to the young.
Medical research 7 hours ago | 4.5 / 5 (2) | 0 |
On May 22, JoVE will publish details of a technique to measure the health of human genetic material in relation to a patient's age. The method is demonstrated by the laboratory of Dr. Gil Atzmon at New York's Albert Einste ...
Medical research 10 hours ago | not rated yet | 0
(Medical Xpress)—Scientists supported by the National Institutes of Health have a new theory as to why a woman's fertility declines after her mid-30s. They also suggest an approach that might help slow ...
Medical research 12 hours ago | not rated yet | 0 |
(HealthDay)—Migraines and depression can each cause a great deal of suffering, but new research indicates the combination of the two may be linked to something else entirely—a smaller brain.
4 hours ago | 5 / 5 (1) | 0 |
Until now, little was scientifically known about the human potential to cultivate compassion—the emotional state of caring for people who are suffering in a way that motivates altruistic behavior.
4 hours ago | 5 / 5 (1) | 2 |
Researchers from Queen Mary, University of London have led the largest sequencing study of human disease to date, investigating the genetic basis of six autoimmune diseases.
7 hours ago | not rated yet | 0 |
Calorie information in fast food restaurants used by 40 percent of 9-18 year olds when making food choices
A new study published online today (Thursday) in the Journal of Public Health has found that of young people who visited fast food or chain restaurants in the U.S. in 2010, girls and youth who were obese were more likely ...
1 hour ago | not rated yet | 0
A new approach for immunizing against influenza elicited a more potent immune response and broader protection than the currently licensed seasonal influenza vaccines when tested in mice and ferrets. The vaccine ...
5 hours ago | not rated yet | 0 |
(HealthDay)—Implementation of systematic monitoring for medication adherence will allow for identification of barriers to adherence and tailoring of interventions, according to a viewpoint piece published ...
3 hours ago | not rated yet | 0