Scientists discover key process that allows colon cancer to metastasize
These are microscopy images of growing colon cancer cells surrounded by stroma cells, mainly fibroblasts (in green). Credit: © E. Batlle lab, IRB Barcelona. Autor: Alexandre Calon
A team of 17 researchers, led by scientists Eduard Batlle and Elena Sancho in the Colorectal Cancer Laboratory at the Institute for Research in Biomedicine (IRB Barcelona), have determined that the ability of colon cancer to metastasize lies in the healthy cells, called stroma, that surround the tumour. Although the stroma has long been hypothesized to be complicit in this process, this study marks the first time that healthy cells in the microenvironment have been observed to play a fundamental role in allowing metastasis to occur in a specific tumour type.
The discovery, which will be tomorrow's Cancer Cell cover story, could translate into direct benefits for patients given that in a little more than five years, tests could be available to predict relapse allowing doctors to target treatment according to prognosis. IRB Barcelona Group Leader Eduard Batlle, ICREA researcher and recipient of an ERC Starting Grant and the Banco Sabadell Biomedical Research Prize, and Associate Researcher Elena Sancho, presented their results at a press conference held during the Barcelona BioMed Conference on "Normal and Tumour Stem Cells", organized by IRB Barcelona and the BBVA Foundation at the Institute d'Estudis Catalans.
Tumour stem cells corrupt healthy cells in tumour microenvironment
By studying 345 cases of colon cancer, using information in public databases and samples of patients provided by three hospitals in Barcelona, the team was able to identify the factors key to colon cancer metastasis. They showed that when tumour stem cells reach the liver, a common target of colon cancer metastasis, they release a molecule called TGF-beta into the microenvironment. The surrounding cells, including macrophages, leukocytes, fibroblasts and endothelial cells, respond by releasing a different set of molecules. The researchers found that the cells in the tumour microenvironment produce interleukin-11 (IL11) and cause a series of genetic changes in the tumour stem cells that allow it to survive in the foreign organ.
"This study proposes a change in paradigm", explains Batlle. "Until now, if we wanted to know whether a colon cancer patient was likely to develop metastasis, we would look at their tumour cells. This study has shown us that, instead of looking at the seed, we need to be looking at the earth. We can predict if a plant will grow if the ground, or substrate, in which the seed is planted is fertilized. TGF-beta is the fertilizer that changes the earth in which the tumour seed grows."
The scientists also observed that tumour cells in the original organ already possess the ability to change their microenvironment. "We can tell whether there will be metastasis through indirect means. If we see that the stroma is already modified in the primary tumour site in the colon, it means that the tumour cells will also be able to change the microenvironment when they disseminate to the liver," explains Alexandre Calon, a French postdoctoral fellow in Batlle's lab and first author of the study.
The epithelial cells of the colon (in blue) form a thin layer that covers the colon. Herein lie the cells which give rise to colon cancer. Credit: © E. Batlle lab, IRB Barcelona. Autor: Alexandre CalonA test to predict relapse ready in five years
Colon cancer is the second cause of cancer deaths worldwide. Current treatment normally combines surgery and chemotherapy. After intervention, patients normally go into remission, which can last months or years. 30-40% will relapse, mostly in the form of metastasis primarily to the liver or to the lung. Elena Sancho explains that "in about five years, we will likely have a test on the market that identifies those patients at risk of metastasis, allowing doctors to fine tune their treatment regimes."
The scientists have observed that about 15% of patients never develop metastasis and this is related to whether or not the stroma has been modified by TGF-beta. This means that armed with a diagnostic test that analyzes the genetic signature of the stroma (whether or not molecules including TGF-beta and interleukin-11 are present), doctors may be able to identify patients at risk of developing metastasis. If the data from this study are confirmed, between 10-15% of patients may no longer require chemotherapy, leading to direct benefits for their health and to a better use of resources. On the other hand, if the test predicts a high risk for metastasis, patients would be able to receive more aggressive treatment and undergo more thorough monitorization.
Validation of scientific results and future treatment for metastasis
The team of researchers also show in the Cancer Cell article that metastasis can be prevented from occurring by eliminating the TGF-beta signal in the stroma. They treated mice with aggressive colon tumours with a TGF-beta inhibitor that is already in clinical trials for other illnesses. Their tumours did not metastasize. "This experiment proves that TGF-beta and the tumour stroma must 'speak to each other' in order for metastasis to occur. Our results in mice also show that patients with activated TGF-beta, and who are in the initial phases of the disease, may benefit from taking a TGF-beta inhibitor", explains Batlle.
As far as the researchers can tell, dependence on TGF-beta is limited to the initial phases of metastasis. Once metastasis takes hold in the foreign organ, the administration of the inhibitor is no longer effective. "Even so, we must point out", says Batlle, "that the development of a drug to treat colon cancer metastasis is a complicated process." Today, the vast majority of inhibitors must first be tested in patients with irreversible prognosis. Clinical trials are designed to slow down tumour growth, while the molecules that we administered to mice don't act on tumour growth but at an earlier step. We have presented our evidence in this article, and we open the door to a future development of a TGF-beta based inhibitor".
This study was made possible thanks to the collaboration of physicians at the Hospital Clinic of Barcelona, the Hospital del Mar, the Hospital de Sant Pau and the group of researchers at the Memorial Sloan Kettering Cancer Center led by Joan Massagué. Massagué, who is also adjunct director of IRB Barcelona and fosters the research on cancer metastasis in the center, participated in the study as an expert on metastasis and TGF-beta.
Other collaborators in the study include David Rossell, Head of the Biostatistics and Bioinformatics Unit at IRB Barcelona, who played a key role in making sense of the enormous amount of genomic data generated in the study, as well as the team of chemist Antoni Riera, also from IRB Barcelona, who contributed work on the TGF-beta inhibitor.
More information: Dependency of Colorectal Cancer on a TGF-beta-Driven Program in Stromal Cells for Metastasis Initiation, Alexandre Calon, Elisa Espinet, Sergio Palomo-Ponce, Daniele V.F. Tauriello, Mar Iglesias, María Virtudes Céspedes, Marta Sevillano, Cristina Nadal, Peter Jung, Xiang H.-F. Zhang, Daniel Byrom, Antoni Riera, David Rossell, Ramon Mangues, Joan Massagué, Elena Sancho, Eduard Batlle. Cancer Cell (2012). November 13 (print issue)
Journal reference: Cancer Cell
- Researchers unravel biochemical factor important in tumor metastasis Nov 09, 2011 | not rated yet | 0
- Researchers discover one of the mechanisms that prevents the spread of colon cancer Sep 30, 2007 | not rated yet | 0
- Stem cells may be key to understanding the origins of colon cancer and detecting relapse Mar 17, 2011 | not rated yet | 0
- Researchers describe a new genetic program that converts static cells into mobile invasive cells Dec 15, 2011 | not rated yet | 0
- A new indicator for breast cancer relapse identified Jun 19, 2012 | not rated yet | 0
- Motion perception revisited: High Phi effect challenges established motion perception assumptions Apr 23, 2013 | 3 / 5 (2) | 2
- Anything you can do I can do better: Neuromolecular foundations of the superiority illusion (Update) Apr 02, 2013 | 4.5 / 5 (11) | 5
- The visual system as economist: Neural resource allocation in visual adaptation Mar 30, 2013 | 5 / 5 (2) | 9
- Separate lives: Neuronal and organismal lifespans decoupled Mar 27, 2013 | 4.9 / 5 (8) | 0
- Sizing things up: The evolutionary neurobiology of scale invariance Feb 28, 2013 | 4.8 / 5 (10) | 14
How can there be a term called "intestinal metaplasia" of stomach
22 hours ago Hello everyone, Ok Stomach's normal epithelium is simple columnar, now in intestinal type of adenocarcinoma of stomach it undergoes "intestinal...
Pressure-volume curve: Elastic Recoil Pressure don't make sense
May 18, 2013 From pressure-volume curve of the lung and chest wall (attached photo), I don't understand why would the elastic recoil pressure of the lung is...
If you became brain-dead, would you want them to pull the plug?
May 17, 2013 I'd want the rest of me to stay alive. Sure it's a lousy way to live but it beats being all-the-way dead. Maybe if I make it 20 years they'll...
MRI bill question
May 15, 2013 Dear PFers, The hospital gave us a $12k bill for one MRI (head with contrast). The people I talked to at the hospital tell me that they do not...
Ratio of Hydrogen of Oxygen in Dessicated Animal Protein
May 13, 2013 As an experiment, for the past few months I've been consuming at least one portion of Jell-O or unflavored Knox gelatin per day. I'm 64, in very...
Alcohol and acetaminophen
May 13, 2013 Edit: sorry for the typo in the title , can't edit I looked around on google quite a bit and it's very hard to find precise information on the...
- More from Physics Forums - Medical Sciences
More news stories
The gap between life expectancy in patients with a mental illness and the general population has widened since 1985 and efforts to reduce this gap should focus on improving physical health, suggest researchers in a paper ...
Cancer 10 hours ago | not rated yet | 0
By studying the roles two proteins, thrombospondin-1 and prosaposin, play in discouraging cancer metastasis, a trans-Atlantic research team has identified a five-amino acid fragment of prosaposin that significantly reduces ...
Cancer 11 hours ago | not rated yet | 0
A novel transcriptome-based classification of colon cancer that improves the current disease stratification based on clinicopathological variables and common DNA markers is presented in a study published in PLOS Medicine this w ...
Cancer 12 hours ago | not rated yet | 0
A study of veterans at high risk for developing lung cancer shows that low-dose computed tomography (LDCT) can be highly effective in helping clinicians spot tiny lung nodules which, in a small number of patients, may indicate ...
Cancer 13 hours ago | not rated yet | 0
An attack on glioblastoma brain tumor cells that uses a modified poliovirus is showing encouraging results in an early study to establish the proper dose level, researchers at Duke Cancer Institute report.
Cancer 15 hours ago | not rated yet | 0
Is it permissible to harm one to save many? Those who tend to say "yes" when faced with this classic dilemma are likely to be deficient in a specific kind of empathy, according to a report published in the scientific journal ...
10 minutes ago | not rated yet | 0 |
Phthalates: Study links chemicals widely found in plastics, processed food to elevated blood pressure in children, teens
Plastic additives known as phthalates (pronounced THAL-ates) are odorless, colorless and just about everywhere: They turn up in flooring, plastic cups, beach balls, plastic wrap, intravenous tubing and—according to the ...
1 hour ago | not rated yet | 0 |
(Medical Xpress)—Native peoples in regions where cameras are uncommon sometimes react with caution when their picture is taken. The fear that something must have been stolen from them to create the photo ...
17 hours ago | 4.2 / 5 (5) | 0 |
(Medical Xpress)—Despite spending billions of dollars on research and development, drug companies have been unable to come up with effective treatments for dementia and Alzheimer's Disease (AD). Now, A. ...
15 hours ago | 4.9 / 5 (13) | 0 |
Australian scientists have charted the path of insulin action in cells in precise detail like never before. This provides a comprehensive blueprint for understanding what goes wrong in diabetes.
17 hours ago | 4.6 / 5 (7) | 0 |