Engrafted stem cell-derived lung organoids that model human lung development

September 11, 2018, Mary Ann Liebert, Inc
Credit: Mary Ann Liebert, Inc., publishers

Researchers have now grown lung organoids from human embryonic stem cells (hESCs) that, after implantation in mice, can develop mature alveolar type 1 (AT1) and AT2 cells and architecture approximating that of human lungs. The strategy is the first to generate both lung distal progenitor cells and mature AT1-like cells. The study is published in Stem Cells and Development.

Ling Yin, Zhili Rong, and colleagues from the Cancer Research Institute of Southern Medical University, Guangzhou, China, present their research in an article titled "Long-Term Engraftment Promotes Differentiation of Alveolar Epithelial Cells from Human Embryonic Stem Cells Derived Lung Organoids". The authors prepared lung organoids by providing hESCs with a specific set of differentiation-inducing growth factors and chemical inhibitors and then embedding these cells in Matrigel. After different growth periods from ~3-6 weeks, formed organoids were engrafted into immunocompromised mice to assess further differentiation. The authors studied these organoids after short-term (21 d) or long-term (?100 d) engraftment and identified a progression from abundant progenitor cell types to more completely differentiated and mature AT1 and AT2 cells as the duration of in vitro differentiation and engraftment increased, thus providing a model of human lung development.

"Using a relatively inexpensive protocol, the authors demonstrate human lung organoids derived from human can generate mature alveolar type I and II -like after four months transplantation in a murine renal capsule model," says Editor-in-Chief Graham C. Parker, Ph.D., The Carman and Ann Adams Department of Pediatrics, Wayne State University School of Medicine, Detroit, MI.

Explore further: Growing kidney tissue simpler and faster will help fight disease

More information: Yong Chen et al, Long-Term Engraftment Promotes Differentiation of Alveolar Epithelial Cells from Human Embryonic Stem Cell Derived Lung Organoids, Stem Cells and Development (2018). DOI: 10.1089/scd.2018.0042

Related Stories

Growing kidney tissue simpler and faster will help fight disease

July 23, 2018
New treatments for kidney diseases and inherited kidney disorders could result from new research that is revolutionising the way human kidney tissue is grown from stem cells.

3-D organoids and RNA sequencing reveal the crosstalk driving lung cell formation

September 7, 2017
To stay healthy, our lungs have to maintain two key populations of cells: the alveolar epithelial cells, which make up the little sacs where gas exchange takes place, and bronchiolar epithelial cells (also known as airway ...

Researchers grow capillaries with a neural organoid

April 10, 2018
A team of researchers at UC Davis has succeeded in growing capillaries on and into a neural organoid. In their paper published in the journal NeuroReport, the group describes how they grew capillaries with the organoid and ...

New study makes strides towards generating lung tissue

May 2, 2017
Using Induced pluripotent stem cells (iPSCs), researchers have for the first time profiled the complete genetic programs of early lung progenitors identifying genes that control lung formation and have created mini-lung organoids ...

Method to create kidney organoids from patient cells provides insights on kidney disease

November 17, 2016
A new method to create kidney organoids from patient cells may provide insights into how kidney diseases arise and how they should be treated. The research will be presented at ASN Kidney Week 2016 November 15¬-20 at McCormick ...

Recommended for you

Separated entry and exit doors for calcium keep energy production smooth in the powerhouses of heart cells

September 18, 2018
Stress demands the heart to work harder and faster. To keep pace, the muscle must make its fuel at an accelerated rate. Bursts of calcium entering mitochondria—the cell's powerhouses—normally help control energy output, ...

First gut bacteria may have lasting effect on ability to fight chronic diseases

September 18, 2018
New research showing that the first bacteria introduced into the gut have a lasting impact may one day allow science to adjust microbiomes—the one-of-a-kind microbial communities that live in our gastrointestinal tracts—to ...

A new defender for your sense of smell

September 18, 2018
New research from the Monell Center increases understanding of a mysterious sensory cell located in the olfactory epithelium, the patch of nasal tissue that contains odor-detecting olfactory receptor cells. The findings suggest ...

Small molecule plays big role in weaker bones as we age

September 18, 2018
With age, expression of a small molecule that can silence others goes way up while a key signaling molecule that helps stem cells make healthy bone goes down, scientists report.

Sperm quality study updates advice for couples trying to conceive

September 17, 2018
Could doctors at fertility clinics be giving men bad advice? Dr. Da Li and Dr. XiuXia Wang, who are clinician-researchers at the Center for Reproductive Medicine of Shengjing Hospital in Shenyang in northeast China, think ...

Antioxidant found to be effective in treating mice with osteoarthritis

September 14, 2018
A team of researchers in Belgium and the Netherlands has found that feeding a common antioxidant to test mice was effective in treating osteoarthritis. In their paper published in Science Translational Medicine, the group ...

0 comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.