Scientists find how to block inflammatory molecules in mouse model of multiple sclerosis

Scientists find how to block inflammatory molecules in mouse model of multiple sclerosis
Fig. 1. REV-ERBα is up-regulated in Th17 cells and inhibits the expression of Th17 signature genes. (A) mRNA expression of REV-ERBα, REV-ERBβ, as well as T cell lineage specifying transcription factors T-bet, Gata3, RORγt, and Foxp3, in naïve T cells and Th0, Th1, Th2, Th17, and iTreg cells differentiated for 3 d in vitro. (B) REV-ERBα and RORγt mRNA expression in Th1, Th17, and iTreg cells over 4 d of in vitro differentiation. (C) Protein expression of REV-ERBα, REV-ERBβ, RORγt, and T-bet during in vitro differentiation of Th1 and Th17 cells over 4 d. (D) mRNA expression of RORγt, T-bet, and REV-ERBα in human CD4+ T cells activated under Th1 and Th17 polarizing conditions for 6 d. (E) FACS analysis of IL-17A and IFN-γ expression in mouse CD4+ T cells activated under Th1 and Th17 polarizing conditions and transduced with MIGR1, REV-ERBα or REV-ERBβ retroviral vectors. Data are representative of 3 independent experiments with triplicate wells for each condition. (F) KEGG pathway analysis of genes differentially expressed in REV-ERBα and MIGR1 retrovirally transduced Th17 cells. (G) Heat map of functional groups of differentially expressed genes in Th17 cells transduced with MIGR1, REV-ERBα, or REV-ERBβ retroviral vectors. Relative fold change was normalized to the average of each row in the matrix. Data represents mean ± SEM. Statistical analyses were performed using unpaired 2-tailed Student’s t test (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).

Your immune system comes ready for battle against bacteria, viruses, fungi and even cancer. But in cases of autoimmune disease, the immune system's superpowers turn it into a supervillain. Now, Salk Institute scientists have discovered a way to stop certain immune system cells from mistakenly attacking the body. Their findings, published the week of August 26, 2019, in the journal Proceedings of the National Academy of Sciences, suggest a new way to target Th17 helper T cells, a type of immune cell that produces interleukin 17, a molecule known to be at the root of autoimmune diseases such as multiple sclerosis, rheumatoid arthritis and psoriasis. Previous efforts targeting Th17 helper T cells have had limited success.

"In an autoimmune disease situation, the turns its weapons on itself," says Associate Professor Ye Zheng, of Salk's NOMIS Center for Immunobiology and Microbial Pathogenesis and co-senior author of the study.

"Autoimmune diseases affect around 1 in 30 people. This research opens the door to treating these patients without triggering harmful side effects," says co-senior author and director of Salk's Gene Expression Laboratory Ronald Evans, who holds the March of Dimes Chair in Molecular and Developmental Biology and is a Howard Hughes Medical investigator.

The team of researchers, including first author Christina Chang, used a mouse model of multiple sclerosis to investigate the overproduction of interleukin 17 in Th17 helper T . These immune cells normally work to cure infections, with interleukin 17 sending an inflammation signal to help kick off the immune response. However, sometimes the become hyperactive and start churning out too much interleukin 17, damaging nearby tissues and kicking off the autoimmune disease process.

Drug treatments can slow interleukin 17 production, but they also work as powerful immunosuppressants, blocking the body's overall ability to fight infections. For the new study, the Salk scientists wondered if there was a way to block only interleukin 17 production without harming immune system function.

The team uncovered a way to use a molecule called REV-ERB to suppress interleukin 17 production. REV-ERB binds to a site on the Th17 helper T cells that is normally occupied by a molecule called ROR, responsible for interleukin 17 production. By taking over the same binding location, REV-ERB competes with ROR. "ROR is a gas pedal that drives these helper T cells, but REV-ERB is like the brakes," says Zheng.

The researchers then showed they were able to chemically activate REV-ERB and delay the onset and slow the progression of symptoms of experimental autoimmune encephalomyelitis, the mouse model of multiple sclerosis.

More work is needed, but the scientists say targeting this particular pathway offers a way to tamp down 17 expression without affecting the rest of the immune system.

"It's an early stage, but it's certainly encouraging," adds Michael Downes, co-author of the study and a senior staff scientist in Salk's Gene Expression Laboratory.

Going forward, the scientists plan to study the role of REV-ERB in models of rheumatoid arthritis and psoriasis.

More information: Christina Chang et al. The nuclear receptor REV-ERBα modulates Th17 cell-mediated autoimmune disease, Proceedings of the National Academy of Sciences (2019). DOI: 10.1073/pnas.1907563116 . … /26/1907563116.short
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Citation: Scientists find how to block inflammatory molecules in mouse model of multiple sclerosis (2019, August 28) retrieved 26 November 2022 from
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