Radiation therapy, macrophages improve efficacy of nanoparticle-delivered cancer therapy

Radiation therapy, macrophages improve efficacy of nanoparticle-delivered cancer therapy
In this illustration based on in vivo microscopy, a tumor-associated macrophage (green) induces a burst of leakage from a tumor blood vessel (red), which releases nanoparticles into the tumor tissue (yellow). The large arrow shows nanoparticles being taken up by the macrophage, while the small dashed arrow depicts how the macrophage elicits further nanoparticle delivery via vascular bursting. Credit: Miles Miller, PhD, and Ralph Weissleder, MD, PhD; Center for Systems Biology, Massachusetts General Hospital

A Massachusetts General Hospital (MGH) research team has identified a surprising new role for the immune cells called macrophages—improving the effectiveness of nanoparticle-delivered cancer therapies. In their Science Translational Medicine report, the investigators describe finding how appropriately timed radiation therapy can improve the delivery of cancer nanomedicines as much as 600 percent by attracting macrophages to tumor blood vessels, which results in a transient "burst" of leakage from capillaries into the tumor.

"The field of nanomedicine has worked to improve selective delivery to tumors for over a decade, typically by engineering ever more advanced nanomaterials and often with mixed clinical success," says lead author Miles Miller, PhD, of the MGH Center for Systems Biology. "Rather than focusing on the nanoparticles themselves, we used in vivo microscopy to discover how to rewire the structure of the tumor itself to more efficiently accumulate a variety of nanomedicines already in clinical use."

Encapsulating cancer drugs in nanoparticles can improve pharmacokinetics—how a drug is absorbed, distributed, metabolized and excreted—by extending a drug's presence in the circulation and avoiding the toxic solvents used in infusion chemotherapy. But in clinical practice, delivering nanoencapsulated drugs into patients' tumors has been challenging, largely because of known factors in the microenvironment of the tumor. High pressures within tumors and low permeability of limit the passage of any drugs from the circulation into .

Intravital imaging movie showing immune cells infiltrating tumors after radiation burst. Credit: M.A. Miller et al., Science Translational Medicine (2017)

A 2015 study by Miller and his colleagues showed that tumor-associated macrophages can improve delivery of nanoparticle-based therapies to tumor cells, and therapy is known to increase the permeability of . But exactly how these effects are produced and how they could be combined to enhance nanomedicine delivery was not known. Answering those questions was the goal of the current study.

Experiments in mouse models of cancer revealed that radiation therapy produced important changes in the tumor microenvironment—including greater blood vessel size and permeability and an increase in the number of macrophages relative to tumor cells. These changes did not appear until 3 to 4 days after administration of radiation therapy and disappeared by day 11. Analysis of patient biopsy samples taken before and several days after radiation therapy for breast or cervical cancer revealed significant macrophage expansion in post-radiation samples, with the greatest increases in patients receiving the highest radiation dosage.

Additional mouse studies showed that, beginning three days after radiation therapy, the uptake of nanoparticles but not of solvent-delivered drugs approximately doubled. High-resolution in vivo microscopy revealed that increases in vascular permeability occurred erratically, with periods of low permeability interrupted by a bursting of vascular contents, including nanoparticles, into the tumors. The rate of bursting increased three days after radiation and was higher on larger blood vessels with adjacent macrophages. Removal of macrophages prevented the radiation-induced changes and the increased uptake of nanoparticles. Combining radiation therapy with cyclophosphamide - a DNA-damaging drug that enhances nanoparticle delivery to tumor cells through similar tumor-priming mechanisms - led to even greater nanoparticle uptake.

Radiation therapy, macrophages improve efficacy of nanoparticle-delivered cancer therapy
Microscopic images of tumor cells (red) before (left) and after (right) radiation, showing how treatment promotes infiltration of immune cells (green) Credit: M.A. Miller et al., Science Translational Medicine (2017)

Testing the therapeutic effect of combining radiation therapy with a nanoparticle-encased chemotherapy drugs in a mouse model confirmed the efficacy of the strategy and the key role of macrophages. While combining radiation with a solvent-based drug had no benefit compared with radiation alone, delivery of a nanoencapsulated version of the same drug three days after radiation eliminated most tumors, an effect that was significantly reduced if macrophages were depleted.

"Finding that this combination of radiation and nanomedicine leads to synergistic tumor eradication in mice provides motivation for clinical trials that combine tumor rewiring using with nanomedicine," says Miller, who is an instructor in Medicine at Harvard Medical School. "Most of the treatments and nanomedicines employed in this study are FDA approved for cancer treatment, so this combination treatment strategy could be tested in clinical trials relatively quickly. And given the role of in this approach, we are particularly interested in combining irradiation and with immuno-oncology therapies."


Explore further

Researchers deliver first 'nanotherapeutics' to tumor

More information: M.A. Miller el al., "Radiation therapy primes tumors for nanotherapeutic delivery via macrophage-mediated vascular bursts," Science Translational Medicine (2017). stm.sciencemag.org/lookup/doi/ … scitranslmed.aal0225
Journal information: Science Translational Medicine

Citation: Radiation therapy, macrophages improve efficacy of nanoparticle-delivered cancer therapy (2017, May 31) retrieved 24 June 2019 from https://medicalxpress.com/news/2017-05-therapy-macrophages-efficacy-nanoparticle-delivered-cancer.html
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.
37 shares

Feedback to editors

User comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more