Spare parts from small parts: Novel scaffolds to grow muscle

Spare parts from small parts: Novel scaffolds to grow muscle
A detail of the muscle tissue that the researchers are replicating using their 3-D material. Credit: RMIT University

Australian biomedical engineers have successfully produced a 3D material that mimics nature to transform cells into muscle.

The researchers have for the first time incorporated the natural processes of embryonic development to build a material that can more naturally communicate with for effective tissue repair.

The breakthrough offers hope to people suffering from injuries where their muscles cannot repair themselves due to trauma or disease, and our rapidly ageing population.

Led by Dr Richard Williams from RMIT University, in Melbourne Australia, the researchers have combined lab-made peptides with natural proteins and polymers to create a 3D "hydrogel scaffold".

The scaffold uses natural mechanisms to incorporate signals found in the natural developmental environment to support and engineer stem cells into .

"Our stem cells are at their best during development, where a scaffold drives them to produce all our tissues and organs; yet as people grow and age, they lose this ability and get a build-up of undesirable structures," Williams said.

"So there is a real need for ways to repair and replace parts of the body as they wear out or become damaged, particularly as our population is now living longer.

"Essentially, we make a scaffold that sends healthy signals to the cells to reorganise themselves to start to grow the small fibres that make up a healthy .

"It's like travelling back in time when our potential for tissue development and repair was at its best."

Williams said it is a "simple process" to deliver complex structures inspired by the way nature pieces structures together during , starting with only two cells.

"We have developed a simple, cheap, yet powerful toolkit that encourages different environments to let different tissues grow," he said.

"The material properties of natural tissues, like muscles, are really complex and extremely difficult to mimic synthetically.

"Researchers have been producing 'organising' signals for years to understand how cells work but only in a flat, or 2D, environment.

"We have found a way to use the proteins present during in a 3D scaffold, and use this extra dimension to potentially grow muscle.

"Our research shows that combining a hydrogel with these small proteins is easy and effective. In fact, just 24 to 72 hours after seeding the cells into the , there was a noticeable reorganisation of into the multi-cellular structures that go on to make up functioning muscle."

The RMIT researchers are now exploring how they can bioprint muscle with the stem already embedded to put nerves, blood vessels and muscles together into lab grown "spare parts".

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More information: Rui Li et al, Large and Small Assembly: Combining Functional Macromolecules with Small Peptides to Control the Morphology of Skeletal Muscle Progenitor Cells, Biomacromolecules (2018). DOI: 10.1021/acs.biomac.7b01632
Journal information: Biomacromolecules

Provided by RMIT University
Citation: Spare parts from small parts: Novel scaffolds to grow muscle (2018, February 20) retrieved 3 October 2022 from
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