Improved retinal transplant technique ready for clinical trials

Improved retinal transplant technique ready for clinical trials
Schematic showing the experimental results. Retinal sheets were grown from wild type human stem cells (left) or human stem cells missing the ISL1 gene (right). On the left, the transplanted photoreceptors (peach cells) are connected to transplanted bipolar cells (green) as part of the retinal sheet. On the right, the graft bipolar cells have died off, and the receptors connect to host bipolar cells (pink). The result is that all ganglion cells on the right respond to light, and responses are higher than those on the left. Photoreceptors (peach cells at the bottom) in the graft connect to bipolar cells (graft bipolar cells: green; host bipolar cells: pink). Host bipolar cells connect to host ganglion cells (top gray cells), which send axons to the brain as the optic nerve. recordings were made from the ganglion cells in response to light (red voltage trace). Credit: RIKEN

Researchers led by Michiko Mandai at the RIKEN Center for Biosystems Dynamics Research (BDR) in Japan have used a genetic modification to improve human-derived retina transplants grown in the lab. After transplant into damaged mouse retinas, timed removal of certain cells from the grafts allowed better connections to host retinas, which resulted in more responsiveness to light in the damaged eyes. Because the retinal sheets were generated from stem cells of human origin, this represents one of the final steps necessary before this technique can be tested in human clinical trials for repairing retinal degeneration. The study was published in the scientific journal iScience.

Retinitis pigmentosa is a hereditary disease in which photoreceptors in the retina die, leaving people with complete loss of vision or progressive loss in certain spots. One promising therapy is to replace the part of the retina at the back of the eye with a new retinal sheet, including the photoreceptors, grown from stem . For this regenerative cell therapy to work, the new light receptors in the graft must connect to neurons in the host retina, allowing light from the outside world to be relayed to the brain, which is how we see.

Based on their previous studies, the team at RIKEN BDR knew that connecting the grafted sheet to in the host is critical. But the retinal sheets naturally contain their own bipolar cells. "Bipolar cells are inevitably born when the retinal sheet develops properly and photoreceptors mature," says Mandai. "But it's their very connection to the bipolar cells in the retinal sheet that prevents the photoreceptors from connecting to the bipolar cells in the host." The solution was to engineer retinal sheets that would lose their bipolar cells during the final stages of maturation.

Improved retinal transplant technique ready for clinical trials
A view of the retina after transplanting a retinal sheet grown from normal human stem cells (top) or the modified stem cells lacking the ISL1 gene (bottom). Bipolar cells are red. Looking at the bottom right image of each panel, we can see that the new type of retinal sheet lacks bipolar cells. This allows transplanted photoreceptors to connect better with host bipolar cells. Credit: RIKEN

The researchers targeted ISLET1, a gene necessary for maturation of bipolar cells that connect to photoreceptors. They began with a line of human stem cells and made clones that lacked the ISLET1 gene. Then, they grew retinal organoid sheets from these clones. Early on, these retinal sheets developed the same way as those grown from normal stem cells. All the different retinal cell types, particularly the photoreceptors, were present and organized in the correct way. As hoped, the targeted bipolar cells eventually died off, which is what happens when they are not allowed to mature.

With this success, the researchers tested their theory by transplanting the new type of retinal sheet into degenerated rat retinas that lacked almost all photoreceptors. A variety of tests showed that the photoreceptors in the retinal sheet matured properly after transplantation, and compared with normal retinal sheets, made better contact with the host eye. To test whether this actually led to better responses to light, the team recorded from , which form the optic nerve and relay visual information from bipolar cells to the brain. Thus, good responses here would mean that more photoreceptors in the graft sheet were connected. As predicted, the response to light was better in these ganglion cells than in those that received a normal retinal sheet transplant.

"The in human stem-cell derived retinas showed a substantial functional improvement compared with the wild-type graft retinas," says Mandai. "Additionally, we were able to make detailed observations of host-graft synapse formation in the absence of graft bipolar cells, which was difficult to do before."

While the group has previously reported similar results using mouse , Mandai says that applying this technique to human cells is a significant milestone. "We can now move forward to applying this strategy in clinical studies," she says. "We expect it will improve clinical outcomes and be useful in general for stem-cell based therapies targeting ."

More information: Suguru Yamasaki et al, A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation, iScience (2021). DOI: 10.1016/j.isci.2021.103657

Journal information: iScience
Provided by RIKEN
Citation: Improved retinal transplant technique ready for clinical trials (2022, January 27) retrieved 21 June 2024 from
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